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A 3D Dynamic In Vitro Model of Inflammatory Tendon Disease

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Specialty Molecular Biology
Date 2021 Mar 9
PMID 33687679
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Abstract

Three-dimensional (3D) cell cultures combining multipotent mesenchymal stromal cells (MSC), tendon extracellular matrix scaffolds, and mechanical stimulation by a bioreactor have been used to induce tenogenic differentiation in vitro. Yet, these conditions alone do not mimic the environment of acute inflammatory tendon disease adequately, thus the results of such studies are not representatives for tendon regeneration after acute injury. In this chapter, we describe two different approaches to introduce inflammatory stimuli, comprising co-culture with leukocytes and supplementation with the cytokines IL-1 β and TNF-α. The presented in vitro model of inflammatory tendon disease could be used to study musculoskeletal pathophysiology and regeneration in more depth.

References
1.
Lui P . Stem cell technology for tendon regeneration: current status, challenges, and future research directions. Stem Cells Cloning. 2015; 8:163-74. PMC: 4685888. DOI: 10.2147/SCCAA.S60832. View

2.
Manning C, Martel C, Sakiyama-Elbert S, Silva M, Shah S, Gelberman R . Adipose-derived mesenchymal stromal cells modulate tendon fibroblast responses to macrophage-induced inflammation in vitro. Stem Cell Res Ther. 2015; 6:74. PMC: 4416344. DOI: 10.1186/s13287-015-0059-4. View

3.
Burk J, Erbe I, Berner D, Kacza J, Kasper C, Pfeiffer B . Freeze-thaw cycles enhance decellularization of large tendons. Tissue Eng Part C Methods. 2013; 20(4):276-84. PMC: 3968887. DOI: 10.1089/ten.TEC.2012.0760. View

4.
Cheng C, Solorio L, Alsberg E . Decellularized tissue and cell-derived extracellular matrices as scaffolds for orthopaedic tissue engineering. Biotechnol Adv. 2014; 32(2):462-84. PMC: 3959761. DOI: 10.1016/j.biotechadv.2013.12.012. View

5.
Youngstrom D, Barrett J . Tendon Differentiation on Decellularized Extracellular Matrix Under Cyclic Loading. Methods Mol Biol. 2016; 1502:195-202. DOI: 10.1007/7651_2016_332. View