Quantum Dots for Improved Single-Molecule Localization Microscopy

Overview

Journal J Phys Chem B

Publisher American Chemical Society

Specialty Chemistry

Date 2021 Mar 8

PMID 33683893

Citations 3

Authors

Jennifer M Urban

Wesley Chiang

Jennetta W Hammond

Nicole M B Cogan

Angela Litzburg

Rebeckah Burke

Harry A Stern

Harris A Gelbard

Bradley L Nilsson

Todd D Krauss

Affiliations

Soon will be listed here.

Abstract

Colloidal semiconductor quantum dots (QDs) have long established their versatility and utility for the visualization of biological interactions. On the single-particle level, QDs have demonstrated superior photophysical properties compared to organic dye molecules or fluorescent proteins, but it remains an open question as to which of these fundamental characteristics are most significant with respect to the performance of QDs for imaging beyond the diffraction limit. Here, we demonstrate significant enhancement in achievable localization precision in QD-labeled neurons compared to neurons labeled with an organic fluorophore. Additionally, we identify key photophysical parameters of QDs responsible for this enhancement and compare these parameters to reported values for commonly used fluorophores for super-resolution imaging.

Citing Articles

Hybrid Amyloid Quantum Dot Nano-Bio Assemblies to Probe Neuroinflammatory Damage.

Chiang W, Urban J, Yanchik-Slade F, Stout A, Hammond J, Nilsson B ACS Chem Neurosci. 2024; 15(17):3124-3135.

PMID: 39146244 PMC: 11378299. DOI: 10.1021/acschemneuro.4c00183.

Quantum Dot Biomimetic for SARS-CoV-2 to Interrogate Blood-Brain Barrier Damage Relevant to NeuroCOVID Brain Inflammation.

Chiang W, Stout A, Yanchik-Slade F, Li H, Terrando N, Nilsson B ACS Appl Nano Mater. 2023; 6(16):15094-15107.

PMID: 37649833 PMC: 10463222. DOI: 10.1021/acsanm.3c02719.

CdS Quantum Dots as Potent Photoreductants for Organic Chemistry Enabled by Auger Processes.

Widness J, Enny D, McFarlane-Connelly K, Miedenbauer M, Krauss T, Weix D J Am Chem Soc. 2022; 144(27):12229-12246.

PMID: 35772053 PMC: 9306379. DOI: 10.1021/jacs.2c03235.

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