The Indiana O'Brien Center for Advanced Renal Microscopic Analysis

Overview

Journal Am J Physiol Renal Physiol

Publisher American Physiological Society

Specialties Nephrology
Physiology

Date 2021 Mar 8

PMID 33682441

Citations 7

Authors

Kenneth W Dunn

Bruce A Molitoris

Pierre C Dagher

Affiliations

Soon will be listed here.

Abstract

The Indiana O'Brien Center for Advanced Microscopic Analysis is a National Institutes of Health (NIH) P30-funded research center dedicated to the development and dissemination of advanced methods of optical microscopy to support renal researchers throughout the world. The Indiana O'Brien Center was founded in 2002 as an NIH P-50 project with the original goal of helping researchers realize the potential of intravital multiphoton microscopy as a tool for understanding renal physiology and pathophysiology. The center has since expanded into the development and implementation of large-scale, high-content tissue cytometry. The advanced imaging capabilities of the center are made available to renal researchers worldwide via collaborations and a unique fellowship program. Center outreach is accomplished through an enrichment core that oversees a seminar series, an informational website, and a biennial workshop featuring hands-on training from members of the Indiana O'Brien Center and imaging experts from around the world.

Citing Articles

Defining the Intravital Renal Disposition of Fluorescence-Quenched Exenatide.

Bryniarski M, Sandoval R, Ruszaj D, Fraser-McArthur J, Yee B, Yacoub R Mol Pharm. 2023; 20(2):987-996.

PMID: 36626167 PMC: 9907348. DOI: 10.1021/acs.molpharmaceut.2c00671.

Defining protein expression in the kidney at large scale: from antibody validation to cytometry analysis.

Sabo A, Winfree S, El-Achkar T Am J Physiol Renal Physiol. 2022; 324(2):F135-F137.

PMID: 36454700 PMC: 9844971. DOI: 10.1152/ajprenal.00262.2022.

The Michigan O'Brien Kidney Research Center: transforming translational kidney research through systems biology.

Bitzer M, Ju W, Subramanian L, Troost J, Tychewicz J, Steck B Am J Physiol Renal Physiol. 2022; 323(4):F401-F410.

PMID: 35924446 PMC: 9485002. DOI: 10.1152/ajprenal.00091.2022.

Intravital Multiphoton Microscopy as a Tool for Studying Renal Physiology, Pathophysiology and Therapeutics.

Molitoris B, Sandoval R, Wagner M Front Physiol. 2022; 13:827280.

PMID: 35399274 PMC: 8988037. DOI: 10.3389/fphys.2022.827280.

Albumin uptake and processing by the proximal tubule: physiological, pathological, and therapeutic implications.

Molitoris B, Sandoval R, Yadav S, Wagner M Physiol Rev. 2022; 102(4):1625-1667.

PMID: 35378997 PMC: 9255719. DOI: 10.1152/physrev.00014.2021.

References

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Adhyapok P, Fu X, Sluka J, Clendenon S, Sluka V, Wang Z . A computational model of liver tissue damage and repair. PLoS One. 2020; 15(12):e0243451. PMC: 7752149. DOI: 10.1371/journal.pone.0243451. View

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Russo L, Sandoval R, Campos S, Molitoris B, Comper W, Brown D . Impaired tubular uptake explains albuminuria in early diabetic nephropathy. J Am Soc Nephrol. 2009; 20(3):489-94. PMC: 2653682. DOI: 10.1681/ASN.2008050503. View

Dunn K, Sandoval R, Molitoris B . Intravital imaging of the kidney using multiparameter multiphoton microscopy. Nephron Exp Nephrol. 2003; 94(1):e7-11. DOI: 10.1159/000070813. View

Clendenon J, Phillips C, Sandoval R, Fang S, Dunn K . Voxx: a PC-based, near real-time volume rendering system for biological microscopy. Am J Physiol Cell Physiol. 2001; 282(1):C213-8. DOI: 10.1152/ajpcell.2002.282.1.C213. View

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10.

Endres B, Sandoval R, Rhodes G, Campos-Bilderback S, Kamocka M, McDermott-Roe C . Intravital imaging of the kidney in a rat model of salt-sensitive hypertension. Am J Physiol Renal Physiol. 2017; 313(2):F163-F173. PMC: 5582897. DOI: 10.1152/ajprenal.00466.2016. View

11.

Fu X, Sluka J, Clendenon S, Dunn K, Wang Z, Klaunig J . Modeling of xenobiotic transport and metabolism in virtual hepatic lobule models. PLoS One. 2018; 13(9):e0198060. PMC: 6136710. DOI: 10.1371/journal.pone.0198060. View

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14.

Chitteti B, Kobayashi M, Cheng Y, Zhang H, Poteat B, Broxmeyer H . CD166 regulates human and murine hematopoietic stem cells and the hematopoietic niche. Blood. 2014; 124(4):519-29. PMC: 4110658. DOI: 10.1182/blood-2014-03-565721. View

15.

Tao W, Rubart M, Ryan J, Xiao X, Qiao C, Hato T . A practical method for monitoring FRET-based biosensors in living animals using two-photon microscopy. Am J Physiol Cell Physiol. 2015; 309(11):C724-35. PMC: 4725439. DOI: 10.1152/ajpcell.00182.2015. View

16.

Dunn K, Fu C, Ho D, Lee S, Han S, Salama P . DeepSynth: Three-dimensional nuclear segmentation of biological images using neural networks trained with synthetic data. Sci Rep. 2019; 9(1):18295. PMC: 6892824. DOI: 10.1038/s41598-019-54244-5. View

17.

Hato T, Winfree S, Day R, Sandoval R, Molitoris B, Yoder M . Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures. J Am Soc Nephrol. 2017; 28(8):2420-2430. PMC: 5533239. DOI: 10.1681/ASN.2016101153. View

18.

Liu X, Wei J, Zhou Q, Molitoris B, Sandoval R, Kobayashi H . Immunotoxin SS1P is rapidly removed by proximal tubule cells of kidney, whose damage contributes to albumin loss in urine. Proc Natl Acad Sci U S A. 2020; 117(11):6086-6091. PMC: 7084118. DOI: 10.1073/pnas.1919038117. View

19.

Lorenz K, Salama P, Dunn K, Delp E . Digital correction of motion artefacts in microscopy image sequences collected from living animals using rigid and nonrigid registration. J Microsc. 2011; 245(2):148-60. PMC: 3856233. DOI: 10.1111/j.1365-2818.2011.03557.x. View

20.

Winfree S, Dagher P, Dunn K, Eadon M, Ferkowicz M, Barwinska D . Quantitative Large-Scale Three-Dimensional Imaging of Human Kidney Biopsies: A Bridge to Precision Medicine in Kidney Disease. Nephron. 2018; 140(2):134-139. PMC: 6165680. DOI: 10.1159/000490006. View