Actually Seeing What Is Going on - Intravital Microscopy in Tissue Engineering

Overview

Journal Front Bioeng Biotechnol

Date 2021 Mar 8

PMID 33681162

Citations 17

Authors

Ravikumar Vaghela

Andreas Arkudas

Raymund E Horch

Maximilian Hessenauer

Affiliations

Soon will be listed here.

Abstract

Intravital microscopy (IVM) study approach offers several advantages over , , and 3D models. IVM provides real-time imaging of cellular events, which provides us a comprehensive picture of dynamic processes. Rapid improvement in microscopy techniques has permitted deep tissue imaging at a higher resolution. Advances in fluorescence tagging methods enable tracking of specific cell types. Moreover, IVM can serve as an important tool to study different stages of tissue regeneration processes. Furthermore, the compatibility of different tissue engineered constructs can be analyzed. IVM is also a promising approach to investigate host reactions on implanted biomaterials. IVM can provide instant feedback for improvising tissue engineering strategies. In this review, we aim to provide an overview of the requirements and applications of different IVM approaches. First, we will discuss the history of IVM development, and then we will provide an overview of available optical modalities including the pros and cons. Later, we will summarize different fluorescence labeling methods. In the final section, we will discuss well-established chronic and acute IVM models for different organs.

Citing Articles

Using Multiphoton Intravital Microscopy to Study Neutrophil Transmigration and Blood-Brain Barrier Permeability in a Mouse Model of Herpes Simplex Virus Encephalitis.

Bricio-Moreno L, Kurt-Jones E, Sorensen E, Luster A, Michael B Methods Mol Biol. 2024; 2828:45-55.

PMID: 39147969 DOI: 10.1007/978-1-0716-4023-4_5.

Impact of Fibrin Gel Architecture on Hepatocyte Growth Factor Release and Its Role in Modulating Cell Behavior for Tissue Regeneration.

Wein S, Jung S, Al Enezy-Ulbrich M, Reicher L, Rutten S, Kuhnel M Gels. 2024; 10(6).

PMID: 38920948 PMC: 11203013. DOI: 10.3390/gels10060402.

Intravital Microscopy for Imaging and Live Cell Tracking of Alveolar Macrophages in Real Time.

Kwak A, Thanabalasuriar A Methods Mol Biol. 2024; 2813:189-204.

PMID: 38888779 DOI: 10.1007/978-1-0716-3890-3_13.

Failure of Autophagy in Pompe Disease.

Do H, Meena N, Raben N Biomolecules. 2024; 14(5).

PMID: 38785980 PMC: 11118179. DOI: 10.3390/biom14050573.

Cytometry in High-Containment Laboratories.

Cohen M, Laux J, Douagi I Methods Mol Biol. 2024; 2779:425-456.

PMID: 38526798 DOI: 10.1007/978-1-0716-3738-8_20.

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