Convergent Antibody Evolution and Clonotype Expansion Following Influenza Virus Vaccination

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2021 Feb 22

PMID 33617543

Citations 14

Authors

David Forgacs

Rodrigo B Abreu

Giuseppe A Sautto

Greg A Kirchenbaum

Elliott Drabek

Kevin S Williamson

Dongkyoon Kim

Daniel E Emerling

Ted M Ross

Affiliations

Soon will be listed here.

Abstract

Recent advances in high-throughput single cell sequencing have opened up new avenues into the investigation of B cell receptor (BCR) repertoires. In this study, PBMCs were collected from 17 human participants vaccinated with the split-inactivated influenza virus vaccine during the 2016-2017 influenza season. A combination of Immune Repertoire Capture (IRCTM) technology and IgG sequencing was performed on ~7,800 plasmablast (PB) cells and preferential IgG heavy-light chain pairings were investigated. In some participants, a single expanded clonotype accounted for ~22% of their PB BCR repertoire. Approximately 60% (10/17) of participants experienced convergent evolution, possessing public PBs that were elicited independently in multiple participants. Binding profiles of one private and three public PBs confirmed they were all subtype-specific, cross-reactive hemagglutinin (HA) head-directed antibodies. Collectively, this high-resolution antibody repertoire analysis demonstrated the impact evolution can have on BCRs in response to influenza virus vaccination, which can guide future universal influenza prophylactic approaches.

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