Strain-level Functional Variation in the Human Gut Microbiota Based on Bacterial Binding to Artificial Food Particles

Overview

Journal Cell Host Microbe

Publisher Cell Press

Specialties Infectious Diseases
Microbiology

Date 2021 Feb 11

PMID 33571448

Citations 19

Authors

Michael L Patnode

Janaki L Guruge

Juan J Castillo

Garret A Couture

Vincent Lombard

Nicolas Terrapon

Bernard Henrissat

Carlito B Lebrilla

Jeffrey I Gordon

Affiliations

Soon will be listed here.

Abstract

Greater understanding of the spatial relationships between members of the human gut microbiota and available nutrients is needed to gain deeper insights about community dynamics and expressed functions. Therefore, we generated a panel of artificial food particles with each type composed of microscopic paramagnetic beads coated with a fluorescent barcode and one of 60 different dietary or host glycan preparations. Analysis of 160 Bacteroides and Parabacteroides strains disclosed diverse strain-specific and glycan-specific binding phenotypes. We identified carbohydrate structures that correlated with binding by specific bacterial strains in vitro and noted strain-specific differences in the catabolism of glycans that mediate adhesion. Mixed in vitro cultures revealed that these adhesion phenotypes are maintained in more complex communities. Additionally, orally administering glycan beads to gnotobiotic mice confirmed specificity in glycan binding. This approach should facilitate analyses of how strains occupying the same physical niche interact, and it should advance the development of synbiotics, more nutritious foods, and microbiota-based diagnostics.

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