Yeast Translation Elongation Factor EEF3 Promotes Late Stages of TRNA Translocation

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2021 Feb 8

PMID 33555093

Citations 16

Authors

Namit Ranjan

Agnieszka A Pochopien

Colin Chih-Chien Wu

Bertrand Beckert

Sandra Blanchet

Rachel Green

Marina V Rodnina

Daniel N Wilson

Affiliations

Soon will be listed here.

Abstract

In addition to the conserved translation elongation factors eEF1A and eEF2, fungi require a third essential elongation factor, eEF3. While eEF3 has been implicated in tRNA binding and release at the ribosomal A and E sites, its exact mechanism of action is unclear. Here, we show that eEF3 acts at the mRNA-tRNA translocation step by promoting the dissociation of the tRNA from the E site, but independent of aminoacyl-tRNA recruitment to the A site. Depletion of eEF3 in vivo leads to a general slowdown in translation elongation due to accumulation of ribosomes with an occupied A site. Cryo-EM analysis of native eEF3-ribosome complexes shows that eEF3 facilitates late steps of translocation by favoring non-rotated ribosomal states, as well as by opening the L1 stalk to release the E-site tRNA. Additionally, our analysis provides structural insights into novel translation elongation states, enabling presentation of a revised yeast translation elongation cycle.

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