Ribosome Profiling Analysis of EEF3-depleted Saccharomyces Cerevisiae

Overview

Journal Sci Rep

Specialty Science

Date 2019 Mar 1

PMID 30816176

Citations 11

Authors

Villu Kasari

Tonu Margus

Gemma C Atkinson

Marcus J O Johansson

Vasili Hauryliuk

Affiliations

Soon will be listed here.

Abstract

In addition to the standard set of translation factors common in eukaryotic organisms, protein synthesis in the yeast Saccharomyces cerevisiae requires an ABCF ATPase factor eEF3, eukaryotic Elongation Factor 3. eEF3 is an E-site binder that was originally identified as an essential factor involved in the elongation stage of protein synthesis. Recent biochemical experiments suggest an additional function of eEF3 in ribosome recycling. We have characterised the global effects of eEF3 depletion on translation using ribosome profiling. Depletion of eEF3 results in decreased ribosome density at the stop codon, indicating that ribosome recycling does not become rate limiting when eEF3 levels are low. Consistent with a defect in translation elongation, eEF3 depletion causes a moderate redistribution of ribosomes towards the 5' part of the open reading frames. We observed no E-site codon- or amino acid-specific ribosome stalling upon eEF3 depletion, supporting its role as a general elongation factor. Surprisingly, depletion of eEF3 leads to a relative decrease in P-site proline stalling, which we hypothesise is a secondary effect of generally decreased translation and/or decreased competition for the E-site with eIF5A.

Citing Articles

The ABCF ATPase New1 resolves translation termination defects associated with specific tRNAArg and tRNALys isoacceptors in the P site.

Turnbull K, Paternoga H, von der Weth E, Egorov A, Pochopien A, Zhang Y Nucleic Acids Res. 2024; 52(19):12005-12020.

PMID: 39217469 PMC: 11514491. DOI: 10.1093/nar/gkae748.

The ABCF ATPase New1 resolves translation termination defects associated with specific tRNA and tRNA isoacceptors in the P site.

Turnbull K, Paternoga H, von der Weth E, Egorov A, Pochopien A, Zhang Y bioRxiv. 2024; .

PMID: 38854126 PMC: 11160720. DOI: 10.1101/2024.05.29.596377.

The ribosome-associated chaperone Zuo1 controls translation upon TORC1 inhibition.

Black A, Williams T, Soubigou F, Joshua I, Zhou H, Lamoliatte F EMBO J. 2023; 42(24):e113240.

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Mitochondrial mRNA localization is governed by translation kinetics and spatial transport.

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