Pluripotent Stem Cell-derived Epithelium Misidentified As Brain Microvascular Endothelium Requires ETS Factors to Acquire Vascular Fate

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2021 Feb 5

PMID 33542154

Citations 100

Authors

Tyler M Lu

Sean Houghton

Tarig Magdeldin

Jose Gabriel Barcia Duran

Andrew P Minotti

Amanda Snead

Andrew Sproul

Duc-Huy T Nguyen

Jenny Xiang

Howard A Fine

Zev Rosenwaks

Lorenz Studer

Shahin Rafii

Dritan Agalliu

David Redmond

Raphael Lis

Affiliations

Soon will be listed here.

Abstract

Cells derived from pluripotent sources in vitro must resemble those found in vivo as closely as possible at both transcriptional and functional levels in order to be a useful tool for studying diseases and developing therapeutics. Recently, differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cells (ECs) with blood-brain barrier (BBB)-like properties has been reported. These cells have since been used as a robust in vitro BBB model for drug delivery and mechanistic understanding of neurological diseases. However, the precise cellular identity of these induced brain microvascular endothelial cells (iBMECs) has not been well described. Employing a comprehensive transcriptomic metaanalysis of previously published hPSC-derived cells validated by physiological assays, we demonstrate that iBMECs lack functional attributes of ECs since they are deficient in vascular lineage genes while expressing clusters of genes related to the neuroectodermal epithelial lineage (Epi-iBMEC). Overexpression of key endothelial ETS transcription factors (, , and ) reprograms Epi-iBMECs into authentic endothelial cells that are congruent with bona fide endothelium at both transcriptomic as well as some functional levels. This approach could eventually be used to develop a robust human BBB model in vitro that resembles the human brain EC in vivo for functional studies and drug discovery.

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