MicroRNA-205-5p Targets the HOXD9-Snail1 Axis to Inhibit Triple Negative Breast Cancer Cell Proliferation and Chemoresistance

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2021 Jan 11

PMID 33428601

Citations 11

Authors

Li-Fen Lin

Yi-Ting Li

Hui Han

Shun-Guo Lin

Affiliations

Soon will be listed here.

Abstract

MicroRNA-205 (miR-205) is believed to be related to the progress of tumors. HOXD9 has been proved to be expressed abnormally in several kinds of cancers. However, the role of miR-205 and HOXD9 in breast cancer remains unclear. The biological role of miR-205 in breast cancer cell proliferation and chemoresistance was investigated. The expression of miR-205 in clinical tissues and breast cancer cell lines were analyzed using quantitative real-time PCR test (qRT-PCR). Overexpression and knockdown models of miR-205 were established to study cell proliferation and chemotherapy-resistant. Moreover, the potential relationships between miR-205 and HOXD9/Snail1 were measured using qRT-PCR, western blot, and chemotherapy-resistant study. miR-205 was lowly expressed in breast cancer tissues and cell lines. Overexpression of miR-205 could inhibit cell proliferation and chemotherapy-resistance. Moreover, we proved that miR-205 could target the HOXD9-Snail1 axis to suppress triple negative breast cancer cell proliferation and chemoresistance. The activation of Snail1 gene by HOXD9 was also proved in this study. The present study may provide a novel insight for the therapeutic strategies of breast cancer through targeting miR-205/HOXD9/Snail1.

Citing Articles

Tumour DNA methylation markers associated with breast cancer survival: a replication study.

Zarean E, Li S, Wong E, Makalic E, Milne R, Giles G Breast Cancer Res. 2025; 27(1):9.

PMID: 39825380 PMC: 11740461. DOI: 10.1186/s13058-024-01955-x.

The regulation of microRNAs on chemoresistance in triple-negative breast cancer: a recent update.

Yan L, Lau A, Xu Y Epigenomics. 2024; .

PMID: 38639712 PMC: 11160456. DOI: 10.2217/epi-2023-0430.

MicroRNAs Associated with Androgen Receptor and Metastasis in Triple-Negative Breast Cancer.

Ahram M, Abu Alragheb B, Abushukair H, Bawadi R, Al-Hussaini M Cancers (Basel). 2024; 16(3).

PMID: 38339416 PMC: 10854913. DOI: 10.3390/cancers16030665.

Overexpression of microRNA-205-5p promotes cholangiocarcinoma growth by reducing expression of homeodomain-interacting protein kinase 3.

Mon A, Intuyod K, Klungsaeng S, Jusakul A, Pongking T, Lert-Itthiporn W Sci Rep. 2023; 13(1):22444.

PMID: 38105269 PMC: 10725890. DOI: 10.1038/s41598-023-49694-x.

HOXD9/miR-451a/PSMB8 axis is implicated in the regulation of cell proliferation and metastasis via PI3K/AKT signaling pathway in human anaplastic thyroid carcinoma.

Zhong Y, Yu F, Yang L, Wang Y, Liu L, Jia C J Transl Med. 2023; 21(1):817.

PMID: 37974228 PMC: 10652604. DOI: 10.1186/s12967-023-04538-0.

References

Ma L, Teruya-Feldstein J, Weinberg R . Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. Nature. 2007; 449(7163):682-8. DOI: 10.1038/nature06174. View

Sekar P, Bharti J, Nigam J, Sharma A, Soni P . Evaluation of p53, HoxD10, and E-Cadherin Status in Breast Cancer and Correlation with Histological Grade and Other Prognostic Factors. J Oncol. 2014; 2014:702527. PMC: 3929168. DOI: 10.1155/2014/702527. View

Batlle E, Sancho E, Franci C, Dominguez D, Monfar M, Baulida J . The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells. Nat Cell Biol. 2000; 2(2):84-9. DOI: 10.1038/35000034. View

Brown M, Tsodikov A, Bauer K, Parise C, Caggiano V . The role of human epidermal growth factor receptor 2 in the survival of women with estrogen and progesterone receptor-negative, invasive breast cancer: the California Cancer Registry, 1999-2004. Cancer. 2008; 112(4):737-47. DOI: 10.1002/cncr.23243. View

Carey L, Winer E, Viale G, Cameron D, Gianni L . Triple-negative breast cancer: disease entity or title of convenience?. Nat Rev Clin Oncol. 2010; 7(12):683-92. DOI: 10.1038/nrclinonc.2010.154. View

Park S, Kwon H, Lee H, Ryu H, Kim S, Kim J . Promoter CpG island hypermethylation during breast cancer progression. Virchows Arch. 2010; 458(1):73-84. DOI: 10.1007/s00428-010-1013-6. View

Shen W, Hu Y, Uttarwar L, Passegue E, Largman C . MicroRNA-126 regulates HOXA9 by binding to the homeobox. Mol Cell Biol. 2008; 28(14):4609-19. PMC: 2447122. DOI: 10.1128/MCB.01652-07. View

Childs G, Fazzari M, Kung G, Kawachi N, Brandwein-Gensler M, McLemore M . Low-level expression of microRNAs let-7d and miR-205 are prognostic markers of head and neck squamous cell carcinoma. Am J Pathol. 2009; 174(3):736-45. PMC: 2665736. DOI: 10.2353/ajpath.2009.080731. View

Wiklund E, Bramsen J, Hulf T, Dyrskjot L, Ramanathan R, Hansen T . Coordinated epigenetic repression of the miR-200 family and miR-205 in invasive bladder cancer. Int J Cancer. 2010; 128(6):1327-34. DOI: 10.1002/ijc.25461. View

10.

Markou A, Tsaroucha E, Kaklamanis L, Fotinou M, Georgoulias V, Lianidou E . Prognostic value of mature microRNA-21 and microRNA-205 overexpression in non-small cell lung cancer by quantitative real-time RT-PCR. Clin Chem. 2008; 54(10):1696-704. DOI: 10.1373/clinchem.2007.101741. View

11.

Bhatnagar N, Li X, Padi S, Zhang Q, Tang M, Guo B . Downregulation of miR-205 and miR-31 confers resistance to chemotherapy-induced apoptosis in prostate cancer cells. Cell Death Dis. 2011; 1:e105. PMC: 3004480. DOI: 10.1038/cddis.2010.85. View

12.

Hakami F, Darda L, Stafford P, Woll P, Lambert D, Hunter K . The roles of HOXD10 in the development and progression of head and neck squamous cell carcinoma (HNSCC). Br J Cancer. 2014; 111(4):807-16. PMC: 4134504. DOI: 10.1038/bjc.2014.372. View

13.

Saha S, Chowdhury R, Mondal N, Roy S, Sengupta S . Expression signatures of HOX cluster genes in cervical cancer pathogenesis: Impact of human papillomavirus type 16 oncoprotein E7. Oncotarget. 2017; 8(22):36591-36602. PMC: 5482679. DOI: 10.18632/oncotarget.16619. View

14.

Chacon R, Costanzo M . Triple-negative breast cancer. Breast Cancer Res. 2010; 12 Suppl 2:S3. PMC: 2972557. DOI: 10.1186/bcr2574. View

15.

Lebanony D, Benjamin H, Gilad S, Ezagouri M, Dov A, Ashkenazi K . Diagnostic assay based on hsa-miR-205 expression distinguishes squamous from nonsquamous non-small-cell lung carcinoma. J Clin Oncol. 2009; 27(12):2030-7. DOI: 10.1200/JCO.2008.19.4134. View

16.

Hu X, Zhang J, Xu B, Cai L, Ragaz J, Wang Z . Cisplatin plus gemcitabine versus paclitaxel plus gemcitabine as first-line therapy for metastatic triple-negative breast cancer (CBCSG006): a randomised, open-label, multicentre, phase 3 trial. Lancet Oncol. 2015; 16(4):436-46. DOI: 10.1016/S1470-2045(15)70064-1. View

17.

Brinckerhoff C, Matrisian L . Matrix metalloproteinases: a tail of a frog that became a prince. Nat Rev Mol Cell Biol. 2002; 3(3):207-14. DOI: 10.1038/nrm763. View

18.

Muenst S, Daster S, Obermann E, Droeser R, Weber W, von Holzen U . Nuclear expression of snail is an independent negative prognostic factor in human breast cancer. Dis Markers. 2013; 35(5):337-44. PMC: 3794547. DOI: 10.1155/2013/902042. View

19.

Li B, Huang Q, Wei G . The Role of HOX Transcription Factors in Cancer Predisposition and Progression. Cancers (Basel). 2019; 11(4). PMC: 6520925. DOI: 10.3390/cancers11040528. View

20.

Wu H, Mo Y . Targeting miR-205 in breast cancer. Expert Opin Ther Targets. 2009; 13(12):1439-48. DOI: 10.1517/14728220903338777. View