Exosomal MiR-205-5p Enhances Angiogenesis and Nasopharyngeal Carcinoma Metastasis by Targeting

Overview

Journal Mol Ther Oncolytics

Publisher Cell Press

Date 2022 Mar 14

PMID 35284624

Authors

Wenjuan Yang

Shiming Tan

Lixia Yang

Xiaohui Chen

Ruiqian Yang

Linda Oyang

Jinguan Lin

Longzheng Xia

Nayiyuan Wu

Yaqian Han

Yanyan Tang

Min Su

Xia Luo

Yiqing Yang

Lisheng Huang

Zifan Hu

Yi Tao

Lin Liu

Yi Jin

Hui Wang

Qianjin Liao

Yujuan Zhou

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to investigate whether and how exosomal miR-205-5p regulated angiogenesis and nasopharyngeal carcinoma (NPC) metastasis. We found that up-regulated serum exosomal miR-205-5p levels were associated with NPC progression and worse overall survival of NPC patients. miR-205-5p over-expression significantly increased tube formation, wound healing, migration and invasion of NPC cells, and lung metastasis of NPC tumors, whereas miR-205-5p inhibition had opposite effects. Exosomal miR-205-5p from NPC cells promoted the migration, tube formation, and microvessel density (MVD) of HUVECs and . Furthermore, bioinformatics-, luciferase reporter-, and biotinylated miR-205-5p-based pull-down assays indicated that miR-205-5p directly bound to the 3' UTR of (). Exosomal miR-205-5p targeted to enhance the EGFR/ERK signaling and MMP2/MMP9 expression, promoting angiogenesis and NPC metastasis, which was abrogated by DSC2 over-expression. Finally, the levels of miR-205-5p transcripts were positively correlated with MVD but negatively with DSC2 expression in NPC tissues, and patients with miR-205/DSC2 NPC had worse overall survival. In conclusion, exosomal miR-205-5p promotes angiogenesis and NPC metastasis by targeting DSC2 to enhance EGFR/ERK signaling and MMP expression. This exosomal/miR-205-5p/EGFR/ERK axis may be a new therapeutic target for intervention of NPC metastasis.

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