Magnesium Sulfate Attenuates Lethality and Oxidative Damage Induced by Different Models of Hypoxia in Mice

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2020 Dec 31

PMID 33381544

Citations 11

Authors

Hamidreza Mohammadi

Amir Shamshirian

Shafagh Eslami

Danial Shamshirian

Mohammad Ali Ebrahimzadeh

Affiliations

Soon will be listed here.

Abstract

Mg is an important cation in our body. It is an essential cofactor for many enzymes. Despite many works, nothing is known about the protective effects of MgSO against hypoxia-induced lethality and oxidative damage in brain mitochondria. In this study, antihypoxic and antioxidative activities of MgSO were evaluated by three experimental models of induced hypoxia (asphyctic, haemic, and circulatory) in mice. Mitochondria protective effects of MgSO were evaluated in mouse brain after induction of different models of hypoxia. Antihypoxic activity was especially pronounced in asphyctic hypoxia, where MgSO at dose 600 mg/kg showed the same activity as phenytoin, which used as a positive control ( < 0.001). In the haemic model, MgSO at all used doses significantly prolonged latency of death. In circulatory hypoxia, MgSO (600 mg/kg) doubles the survival time. MgSO significantly decreased lipid peroxidation and protein carbonyl and improved mitochondrial function and glutathione content in brain mitochondria compared to the control groups. The results obtained in this study showed that MgSO administration has protective effects against lethality induced by different models of hypoxia and improves brain mitochondria oxidative damage.

Citing Articles

Magnesium (Mg): Essential Mineral for Neuronal Health: From Cellular Biochemistry to Cognitive Health and Behavior Regulation.

Kumar A, Mehan S, Tiwari A, Khan Z, Gupta G, Narula A Curr Pharm Des. 2024; 30(39):3074-3107.

PMID: 39253923 DOI: 10.2174/0113816128321466240816075041.

Effects of mid‑gestational sevoflurane and magnesium sulfate on maternal oxidative stress, inflammation and fetal brain histopathology.

Ozdemi R C, Isik B, Koca G, Inan M Exp Ther Med. 2024; 28(1):286.

PMID: 38827470 PMC: 11140313. DOI: 10.3892/etm.2024.12574.

Magnesium sulfate and risk of hypoxic-ischemic encephalopathy in a high-risk cohort.

Minor K, Liu J, Druzin M, El-Sayed Y, Hintz S, Bonifacio S Am J Obstet Gynecol. 2024; 231(6):647.e1-647.e12.

PMID: 38580044 PMC: 11508778. DOI: 10.1016/j.ajog.2024.04.001.

Synthesis of an insulin-loaded mucoadhesive nanoparticle designed for intranasal administration: focus on new diffusion media.

Jamshidnejad-Tosaramandani T, Kashanian S, Karimi I, Schioth H Front Pharmacol. 2023; 14:1227423.

PMID: 37701036 PMC: 10494546. DOI: 10.3389/fphar.2023.1227423.

Bloodletting Acupuncture at Jing-Well Points on Hand Induced Autophagy to Alleviate Brain Injury in Acute Altitude Hypoxic Rats by Activating PINK1/Parkin Pathway.

Li Y, Li M, Wang C, Li Y, Sa Y, Guo Y Chin J Integr Med. 2023; 29(10):932-940.

PMID: 37434031 DOI: 10.1007/s11655-023-3597-0.

References

MacNee W, Rahman I . Oxidants and antioxidants as therapeutic targets in chronic obstructive pulmonary disease. Am J Respir Crit Care Med. 1999; 160(5 Pt 2):S58-65. DOI: 10.1164/ajrccm.160.supplement_1.15. View

Chiarello D, Marin R, Proverbio F, Benzo Z, Pinero S, Botana D . Effect of hypoxia on the calcium and magnesium content, lipid peroxidation level, and Ca²⁺-ATPase activity of syncytiotrophoblast plasma membranes from placental explants. Biomed Res Int. 2014; 2014:597357. PMC: 4142282. DOI: 10.1155/2014/597357. View

Regan R, Jasper E, Guo Y, Panter S . The effect of magnesium on oxidative neuronal injury in vitro. J Neurochem. 1998; 70(1):77-85. DOI: 10.1046/j.1471-4159.1998.70010077.x. View

Pilchova I, Klacanova K, Tatarkova Z, Kaplan P, Racay P . The Involvement of Mg in Regulation of Cellular and Mitochondrial Functions. Oxid Med Cell Longev. 2017; 2017:6797460. PMC: 5516748. DOI: 10.1155/2017/6797460. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Steenbergen C, Murphy E, Watts J, London R . Correlation between cytosolic free calcium, contracture, ATP, and irreversible ischemic injury in perfused rat heart. Circ Res. 1990; 66(1):135-46. DOI: 10.1161/01.res.66.1.135. View

Harman A, Nieminen A, Lemasters J, Herman B . Cytosolic free magnesium, ATP and blebbing during chemical hypoxia in cultured rat hepatocytes. Biochem Biophys Res Commun. 1990; 170(2):477-83. DOI: 10.1016/0006-291x(90)92116-h. View

Maiti P, Singh S, Sharma A, Muthuraju S, Banerjee P, Ilavazhagan G . Hypobaric hypoxia induces oxidative stress in rat brain. Neurochem Int. 2006; 49(8):709-16. DOI: 10.1016/j.neuint.2006.06.002. View

Spencer J . Beyond antioxidants: the cellular and molecular interactions of flavonoids and how these underpin their actions on the brain. Proc Nutr Soc. 2010; 69(2):244-60. DOI: 10.1017/S0029665110000054. View

10.

Mishra O, Zubrow A, Ashraf Q . Nitric oxide-mediated activation of extracellular signal-regulated kinase (ERK) and c-jun N-terminal kinase (JNK) during hypoxia in cerebral cortical nuclei of newborn piglets. Neuroscience. 2003; 123(1):179-86. DOI: 10.1016/j.neuroscience.2003.08.008. View

11.

Garcia L, Dejong S, Martin S, Smith R, Buettner G, Kerber R . Magnesium reduces free radicals in an in vivo coronary occlusion-reperfusion model. J Am Coll Cardiol. 1998; 32(2):536-9. DOI: 10.1016/s0735-1097(98)00231-9. View

12.

Maulik D, Zanelli S, Numagami Y, Ohnishi S, Mishra O, Delivoria-Papadopoulos M . Oxygen free radical generation during in-utero hypoxia in the fetal guinea pig brain: the effects of maturity and of magnesium sulfate administration. Brain Res. 1999; 817(1-2):117-22. DOI: 10.1016/s0006-8993(98)01235-9. View

13.

Spandou E, Soubasi V, Papoutsopoulou S, Augoustides-Savvopoulou P, Loizidis T, Pazaiti A . Neuroprotective effect of long-term MgSO4 administration after cerebral hypoxia-ischemia in newborn rats is related to the severity of brain damage. Reprod Sci. 2007; 14(7):667-77. DOI: 10.1177/1933719107305864. View

14.

Bakonyi T, Radak Z . High altitude and free radicals. J Sports Sci Med. 2014; 3(2):64-9. PMC: 3899533. View

15.

Gunther T, Vormann J, Hollriegl V . Effects of magnesium and iron on lipid peroxidation in cultured hepatocytes. Mol Cell Biochem. 1995; 144(2):141-5. DOI: 10.1007/BF00944393. View

16.

Mohammadi H, Karimi G, Rezayat S, Dehpour A, Shafiee H, Nikfar S . Benefit of nanocarrier of magnetic magnesium in rat malathion-induced toxicity and cardiac failure using non-invasive monitoring of electrocardiogram and blood pressure. Toxicol Ind Health. 2011; 27(5):417-29. DOI: 10.1177/0748233710387634. View

17.

Sameshima H, Ota A, Ikenoue T . Pretreatment with magnesium sulfate protects against hypoxic-ischemic brain injury but postasphyxial treatment worsens brain damage in seven-day-old rats. Am J Obstet Gynecol. 1999; 180(3 Pt 1):725-30. DOI: 10.1016/s0002-9378(99)70279-6. View

18.

Abad C, Teppa-Garran A, Proverbio T, Pinero S, Proverbio F, Marin R . Effect of magnesium sulfate on the calcium-stimulated adenosine triphosphatase activity and lipid peroxidation of red blood cell membranes from preeclamptic women. Biochem Pharmacol. 2005; 70(11):1634-41. DOI: 10.1016/j.bcp.2005.09.009. View

19.

Shechter M . Magnesium and cardiovascular system. Magnes Res. 2010; 23(2):60-72. DOI: 10.1684/mrh.2010.0202. View

20.

Peruche B, Ahlemeyer B, Brungs H, Krieglstein J . Cultured neurons for testing antihypoxic drug effects. J Pharmacol Methods. 1990; 23(1):63-77. DOI: 10.1016/0160-5402(90)90009-a. View