A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the "Hook Effect"

Overview

Journal J Anal Methods Chem

Publisher Wiley

Specialty Chemistry

Date 2020 Dec 30

PMID 33376621

Citations 2

Authors

Shuang Han

Yifeng Xue

Junlan Zhang

Jianrong Huang

Xiuxia Liu

Yankun Yang

Zhonghu Bai

Chunxin Wang

Affiliations

Soon will be listed here.

Abstract

A chemiluminescent immunoassay for human serum osteocalcin, or bone Gla protein, was established using a double-antibody sandwich model. Examination of the hook effect revealed that it was significantly reduced, and no hook effect was observed at an osteocalcin concentration of 4000 ng/mL. The established method showed good analytical performance and thermal stability. The limit of detection was 0.03 ng/mL. The intra-assay coefficient of variation (CV) was 3.22%-5.64%, the interassay CV was 4.42%-7.25%, and the recovery rate was 93.22%-107.99%. Cross-reactivity (CR) was not observed with bovine, rat, mouse, rabbit, or porcine samples. The developed method showed a good correlation with the N-MID product from Roche. In total, 1069 clinical patient samples were measured using the reagent kit developed in this study.

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