Packaging Recombinant DNA Molecules into Bacteriophage Particles in Vitro

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Aug 1

PMID 333431

Citations 156

Authors

B Hohn

K Murray

Affiliations

Soon will be listed here.

Abstract

Recombinant phage genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro. The process is efficient and nonselective and offers containment in initial stages of handling recombinant DNA. Ligase [poly(deoxyribonucleotide):poly-(deoxyribonucleotide) ligase (AMP-forming), EC 6.5.1.1] reaction products can recombine with endogenous phage DNA during packaging, but UV-irradiation eliminates the biological activity of the endogenous DNA.

Citing Articles

Transmission Dynamics and Novel Treatments of High Risk Carbapenem-Resistant : The Lens of One Health.

Zhu J, Chen T, Ju Y, Dai J, Zhuge X Pharmaceuticals (Basel). 2024; 17(9).

PMID: 39338368 PMC: 11434721. DOI: 10.3390/ph17091206.

Environmental DNA transformation resulted in an active phage in Escherichia coli.

Karaynir A, Bozdogan B, Salih Dogan H PLoS One. 2023; 18(10):e0292933.

PMID: 37831666 PMC: 10575539. DOI: 10.1371/journal.pone.0292933.

Phage therapy: From biological mechanisms to future directions.

Strathdee S, Hatfull G, Mutalik V, Schooley R Cell. 2023; 186(1):17-31.

PMID: 36608652 PMC: 9827498. DOI: 10.1016/j.cell.2022.11.017.

Rapid and Accurate Assembly of Large DNA Assisted by Packaging of Bacteriophage.

Nozaki S ACS Synth Biol. 2022; 11(12):4113-4122.

PMID: 36446634 PMC: 9764419. DOI: 10.1021/acssynbio.2c00419.

Hybrid Vigor: Importance of Hybrid λ Phages in Early Insights in Molecular Biology.

Feiss M, Young R, Ramsey J, Adhya S, Georgopoulos C, Hendrix R Microbiol Mol Biol Rev. 2022; 86(4):e0012421.

PMID: 36165780 PMC: 9799177. DOI: 10.1128/mmbr.00124-21.

References

Hohn T, Wurtz M, Hohn B . Capsid transformation during packaging of bacteriophage lambdaDNA. Philos Trans R Soc Lond B Biol Sci. 1976; 276(943):51-61. DOI: 10.1098/rstb.1976.0097. View

Sternberg N, Tiemeier D, Enquist L . In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1. Gene. 1977; 1(3-4):255-80. DOI: 10.1016/0378-1119(77)90049-x. View

Ratzkin B, Carbon J . Functional expression of cloned yeast DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1977; 74(2):487-91. PMC: 392314. DOI: 10.1073/pnas.74.2.487. View

Murray N, Brammar W, Murray K . Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet. 1977; 150(1):53-61. DOI: 10.1007/BF02425325. View

Morrow J, Cohen S, Chang A, Boyer H, Goodman H, Helling R . Replication and transcription of eukaryotic DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1974; 71(5):1743-7. PMC: 388315. DOI: 10.1073/pnas.71.5.1743. View

Gottesman S, Gottesman M . Excision of prophage lambda in a cell-free system. Proc Natl Acad Sci U S A. 1975; 72(6):2188-92. PMC: 432722. DOI: 10.1073/pnas.72.6.2188. View

Sharp P, Sugden B, Sambrook J . Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973; 12(16):3055-63. DOI: 10.1021/bi00740a018. View

Chang A, Lansman R, Clayton D, Cohen S . Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids. Cell. 1975; 6(2):231-44. DOI: 10.1016/0092-8674(75)90014-8. View

Cameron J, Panasenko S, Lehman I, Davis R . In vitro construction of bacteriophage lambda carrying segments of the Escherichia coli chromosome: selection of hybrids containing the gene for DNA ligase. Proc Natl Acad Sci U S A. 1975; 72(9):3416-20. PMC: 433004. DOI: 10.1073/pnas.72.9.3416. View

10.

Nathans D, Smith H . Restriction endonucleases in the analysis and restructuring of dna molecules. Annu Rev Biochem. 1975; 44:273-93. DOI: 10.1146/annurev.bi.44.070175.001421. View

11.

Murray N, Murray K . Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments. Nature. 1974; 251(5475):476-81. DOI: 10.1038/251476a0. View

12.

LEDERBERG E, Cohen S . Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol. 1974; 119(3):1072-4. PMC: 245717. DOI: 10.1128/jb.119.3.1072-1074.1974. View

13.

Chang A, Cohen S . Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli. Proc Natl Acad Sci U S A. 1974; 71(4):1030-4. PMC: 388155. DOI: 10.1073/pnas.71.4.1030. View

14.

Hohn B, Hohn T . Activity of empty, headlike particles for packaging of DNA of bacteriophage lambda in vitro. Proc Natl Acad Sci U S A. 1974; 71(6):2372-6. PMC: 388457. DOI: 10.1073/pnas.71.6.2372. View

15.

Syvanen M . In vitro genetic recombination of bacteriophage lambda. Proc Natl Acad Sci U S A. 1974; 71(6):2496-9. PMC: 388486. DOI: 10.1073/pnas.71.6.2496. View

16.

Casjens S, Horn T, Kaiser A . Head assembly steps controlled by genes F and W in bacteriophage lambda. J Mol Biol. 1972; 64(3):551-63. DOI: 10.1016/0022-2836(72)90082-4. View

17.

Mandel M, Higa A . Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970; 53(1):159-62. DOI: 10.1016/0022-2836(70)90051-3. View

18.

Weiss B, Richardson C . Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage. Proc Natl Acad Sci U S A. 1967; 57(4):1021-8. PMC: 224649. DOI: 10.1073/pnas.57.4.1021. View

19.

Borck K, Beggs J, Brammar W, Hopkins A, Murray N . The construction in vitro of transducing derivatives of phage lambda. Mol Gen Genet. 1976; 146(2):199-207. DOI: 10.1007/BF00268089. View

20.

Kedes L, Chang A, Houseman D, Cohen S . Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli. Nature. 1975; 255(5509):533-8. DOI: 10.1038/255533a0. View