An Intron in a Ribosomal Protein Gene from Tetrahymena

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1986 Oct 1

PMID 16453715

Citations 13

Authors

H Nielsen

P H Andreasen

H Dreisig

K Kristiansen

J Engberg

Affiliations

Soon will be listed here.

Abstract

We have cloned and sequenced a single copy gene encoding a ribosomal protein from the ciliate Tetrahymena thermophila. The gene product was identified as ribosomal protein S25 by comparison of the migration in two-dimensional polyacrylamide gels of the protein synthesized by translation in vitro of hybrid-selected mRNA and authentic ribosomal proteins. The proteins show strong homology to ribosomal protein S12 from Escherichia coli. The coding region of the gene is interrupted by a 979-bp intron 68 bp downstream of the translation start. This is the first intron in a protein encoding gene of a ciliate to be described at the nucleotide sequence level. The intron obeys the GT/AG rule for splice junctions of nuclear mRNA introns from higher eukaryotes but lacks the pyrimidine stretch usually found in the immediate vicinity of the 3' splice junction. The structure of the intron and the fact that it is found together with the well described self-splicing rRNA intron is discussed in relation to the evolution of RNA splicing.

Citing Articles

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The DNA of ciliated protozoa.

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Alterations in ribosomal protein RPS28 can diversely affect translational accuracy in Saccharomyces cerevisiae.

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Unusual ciliate-specific codons in Tetrahymena mRNAs are translated correctly in a rabbit reticulocyte lysate supplemented with a subcellular fraction from Tetrahymena.

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