3-D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening

Overview

Journal ACS Biomater Sci Eng

Publisher American Chemical Society

Specialties Biomedical Engineering
Biotechnology

Date 2020 Dec 15

PMID 33320634

Citations 15

Authors

Haibin Guo

Nan Deng

Lei Dou

Huifen Ding

Tracy Criswell

Anthony Atala

Cristina M Furdui

Yuanyuan Zhang

Affiliations

Soon will be listed here.

Abstract

The development of human cell-based systems to replace the use of rodents or the two-dimensional culture of cells for studying nephrotoxicity is urgently needed. Human urine-derived stem cells were differentiated into renal tubular epithelial cells in three-dimensional (3-D) culture after being induced by a kidney extracellular matrix. Levels of CYP2E1 and KIM-1 in 3-D organoids were significantly increased in response to acetone and cisplatin. This 3-D culture system provides an alternative tool for nephrotoxicity screening and research.

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