Developmentally Regulated Novel Non-coding Anti-sense Regulators of MRNA Translation in

Overview

Journal iScience

Publisher Cell Press

Date 2020 Dec 9

PMID 33294788

Citations 9

Authors

K Shanmugha Rajan

Tirza Doniger

Smadar Cohen-Chalamish

Praveenkumar Rengaraj

Beathrice Galili

Saurav Aryal

Ron Unger

Christian Tschudi

Shulamit Michaeli

Affiliations

Soon will be listed here.

Abstract

The parasite is the causative agent of sleeping sickness and cycles between insect and mammalian hosts. The parasite appears to lack conventional transcriptional regulation of protein coding genes, and mRNAs are processed from polycistronic transcripts by the concerted action of splicing and polyadenylation. Regulation of mRNA function is mediated mainly by RNA binding proteins affecting mRNA stability and translation. In this study, we describe the identification of 62 non-coding (nc) RNAs that are developmentally regulated and/or respond to stress. We characterized two novel anti-sense RNA regulators (TBsRNA-33 and 37) that originate from the rRNA loci, associate with ribosomes and polyribosomes, and interact with distinct mRNA species to regulate translation. Thus, this study suggests for the first-time anti-sense RNA regulators as an additional layer for controlling gene expression in these parasites.

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