A Dual-labeling Probe to Track Functional Mitochondria-lysosome Interactions in Live Cells

Overview

Journal Nat Commun

Specialty Biology

Date 2020 Dec 9

PMID 33293545

Citations 42

Authors

Qixin Chen

Hongbao Fang

Xintian Shao

Zhiqi Tian

Shanshan Geng

Yuming Zhang

Huaxun Fan

Pan Xiang

Jie Zhang

Xiaohe Tian

Kai Zhang

Weijiang He

Zijian Guo

Jiajie Diao

Affiliations

Soon will be listed here.

Abstract

Mitochondria-lysosome interactions are essential for maintaining intracellular homeostasis. Although various fluorescent probes have been developed to visualize such interactions, they remain unable to label mitochondria and lysosomes simultaneously and dynamically track their interaction. Here, we introduce a cell-permeable, biocompatible, viscosity-responsive, small organic molecular probe, Coupa, to monitor the interaction of mitochondria and lysosomes in living cells. Through a functional fluorescence conversion, Coupa can simultaneously label mitochondria with blue fluorescence and lysosomes with red fluorescence, and the correlation between the red-blue fluorescence intensity indicates the progress of mitochondria-lysosome interplay during mitophagy. Moreover, because its fluorescence is sensitive to viscosity, Coupa allowed us to precisely localize sites of mitochondria-lysosome contact and reveal increases in local viscosity on mitochondria associated with mitochondria-lysosome contact. Thus, our probe represents an attractive tool for the localization and dynamic tracking of functional mitochondria-lysosome interactions in living cells.

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