G9a/GLP-sensitivity of H3K9me2 Demarcates Two Types of Genomic Compartments

Overview

Journal Genomics Proteomics Bioinformatics

Specialty Biology

Date 2020 Dec 7

PMID 33285284

Citations 6

Authors

Zixiang Yan

Luzhang Ji

Xiangru Huo

Qianfeng Wang

Yuwen Zhang

Bo Wen

Affiliations

Soon will be listed here.

Abstract

In the nucleus, chromatin is folded into hierarchical architecture that is tightly linked to various nuclear functions. However, the underlying molecular mechanisms that confer these architectures remain incompletely understood. Here, we investigated the functional roles of H3 lysine 9 dimethylation (H3K9me2), one of the abundant histone modifications, in three-dimensional (3D) genome organization. Unlike in mouse embryonic stem cells, inhibition of methyltransferases G9a and GLP in differentiated cells eliminated H3K9me2 predominantly at A-type (active) genomic compartments, and the level of residual H3K9me2 modifications was strongly associated with B-type (inactive) genomic compartments. Furthermore, chemical inhibition of G9a/GLP in mouse hepatocytes led to decreased chromatin-nuclear lamina interactions mainly at G9a/GLP-sensitive regions, increased degree of genomic compartmentalization, and up-regulation of hundreds of genes that were associated with alterations of the 3D chromatin. Collectively, our data demonstrated essential roles of H3K9me2 in 3D genome organization.

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