A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2020 Dec 5

PMID 33278361

Citations 7

Authors

Romain Forey

Antoine Barthe

Mireille Tittel-Elmer

Maxime Wery

Marie-Benedicte Barrault

Cecile Ducrot

Andrew Seeber

Nils Krietenstein

Ugo Szachnowski

Magdalena Skrzypczak

Krzysztof Ginalski

Maga Rowicka

Jennifer A Cobb

Oliver J Rando

Julie Soutourina

Michel Werner

Karine Dubrana

Susan M Gasser

Antonin Morillon

Philippe Pasero

Armelle Lengronne

Jerome Poli

Affiliations

Soon will be listed here.

Abstract

Mre11-Rad50-Xrs2 (MRX) is a highly conserved complex with key roles in various aspects of DNA repair. Here, we report a new function for MRX in limiting transcription in budding yeast. We show that MRX interacts physically and colocalizes on chromatin with the transcriptional co-regulator Mediator. MRX restricts transcription of coding and noncoding DNA by a mechanism that does not require the nuclease activity of Mre11. MRX is required to tether transcriptionally active loci to the nuclear pore complex (NPC), and it also promotes large-scale gene-NPC interactions. Moreover, MRX-mediated chromatin anchoring to the NPC contributes to chromosome folding and helps to control gene expression. Together, these findings indicate that MRX has a role in transcription and chromosome organization that is distinct from its known function in DNA repair.

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