Matrix Metalloproteinase-2, -7, and -9 Activities in Dogs with Idiopathic Pulmonary Fibrosis Compared to Healthy Dogs and Dogs with Other Respiratory Diseases

Overview

Journal J Vet Intern Med

Specialties General Medicine
Veterinary Medicine

Date 2020 Dec 4

PMID 33274549

Citations 5

Authors

Merita Maatta

Henna P Laurila

Saila Holopainen

Kaisa Aaltonen

Liisa Lilja-Maula

Sanna Viitanen

Minna M Rajamaki

Affiliations

Soon will be listed here.

Abstract

Background: Canine idiopathic pulmonary fibrosis (CIPF) is a chronic, interstitial lung disease that mainly affects West Highland white terriers (WHWTs) and is characterized by excessive deposition of extracellular matrix (ECM) in the lung. Matrix metalloproteinases (MMPs) participate in remodeling of ECM.

Objectives: To compare metalloproteinase-2, -7 and -9 activities in blood or bronchoalveolar lavage fluid (BALF) samples or both of CIPF WHWTs with healthy WHWTs, healthy dogs of other breeds, and dogs with other lung diseases and determine if these MMPs could be used as diagnostic and prognostic markers for CIPF.

Animals: Forty-four CIPF WHWTs, 24 dogs with chronic bronchitis (CB), 17 with eosinophilic bronchopneumopathy (EBP), 10 with bacterial pneumonia, 39 healthy WHWTs, and 35 healthy dogs of other breeds.

Methods: Cross-sectional observational study. Pro-MMP and active MMP activities were analyzed by zymography.

Results: In serum, significantly higher (P < .01) pro-MMP-7 activities were observed in CIPF WHWTs compared to healthy dogs of other breeds, dogs with CB and dogs with EBP. In BALF of CIPF WHWTs, both pro-MMP-9 and pro-MMP-2 activities were significantly higher (P < .01) compared to healthy WHWTs, but these differences were not detected in plasma. The CIPF WHWTs had significantly higher (P < .05) activities of pro-MMP-9 compared to dogs with CB and of pro-MMP-2 compared to dogs with CB and EBP. No statistically significant prognostic factors were observed in CIPF WHWTs.

Conclusions And Clinical Relevance: Serum MMP-7 and BALF MMP-2 and -9 potentially may be useful diagnostic markers but not prognostic markers for CIPF.

Citing Articles

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Osteopontin and fibronectin in lung tissue, serum, and bronchoalveolar lavage fluid of dogs with idiopathic pulmonary fibrosis and control dogs.

Fastres A, Roels E, Tutunaru A, Bolen G, Merveille A, Day M J Vet Intern Med. 2023; 37(6):2468-2477.

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Inoue R, Yasuma T, DAlessandro V, Toda M, Ito T, Tomaru A Int J Mol Sci. 2023; 24(7).

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Polycythemia in dogs with chronic hypoxic pulmonary disease.

Holopainen S, Laurila H, Lappalainen A, Rajamaki M, Viitanen S J Vet Intern Med. 2022; 36(4):1202-1210.

PMID: 35702817 PMC: 9308418. DOI: 10.1111/jvim.16466.

Matrix metalloproteinase-2, -7, and -9 activities in dogs with idiopathic pulmonary fibrosis compared to healthy dogs and dogs with other respiratory diseases.

Maatta M, Laurila H, Holopainen S, Aaltonen K, Lilja-Maula L, Viitanen S J Vet Intern Med. 2020; 35(1):462-471.

PMID: 33274549 PMC: 7848316. DOI: 10.1111/jvim.15970.

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