Digital PCR: What Relevance to Plant Studies?

Overview

Journal Biology (Basel)

Publisher MDPI

Specialty Biology

Date 2020 Dec 3

PMID 33266157

Citations 14

Authors

Caterina Morcia

Roberta Ghizzoni

Chiara Delogu

Lorella Andreani

Paola Carnevali

Valeria Terzi

Affiliations

Soon will be listed here.

Abstract

Digital PCR (dPCR) is a breakthrough technology that able to provide sensitive and absolute nucleic acid quantification. It is a third-generation technology in the field of nucleic acid amplification. A unique feature of the technique is that of dividing the sample into numerous separate compartments, in each of which an independent amplification reaction takes place. Several instrumental platforms have been developed for this purpose, and different statistical approaches are available for reading the digital output data. The dPCR assays developed so far in the plant science sector were identified in the literature, and the major applications, advantages, disadvantages, and applicative perspectives of the technique are presented and discussed in this review.

Citing Articles

Droplet Digital PCR: A New Molecular Method to Detect G1105S/V Mutations in Gene.

Sanchez-Zelaia H, Nanni I, Oggiano I, Hernandez M, Diez-Navajas A, Collina M Biology (Basel). 2024; 13(11).

PMID: 39596874 PMC: 11592065. DOI: 10.3390/biology13110919.

Early Detection of Both f. and f. in Asymptomatic Barley Leaves Using Digital Droplet PCR (ddPCR).

Bouhouch Y, Aggad D, Richet N, Rehman S, Al-Jaboobi M, Kehel Z Int J Mol Sci. 2024; 25(22).

PMID: 39596050 PMC: 11593351. DOI: 10.3390/ijms252211980.

Towards Pathogen-Free Coconut Germplasm Exchange.

Yang C, Nguyen V, Nulu N, Kalaipandian S, Beveridge F, Biddle J Plants (Basel). 2024; 13(13).

PMID: 38999649 PMC: 11244555. DOI: 10.3390/plants13131809.

Assessment of Common Factors Associated with Droplet Digital PCR (ddPCR) Quantification of in Soil.

Lawaju B, Yan G Int J Mol Sci. 2024; 25(6).

PMID: 38542075 PMC: 10970607. DOI: 10.3390/ijms25063104.

Improved Canker Processing and Viability Droplet Digital PCR Allow Detection of Viable Nonculturable Cells in Apple Bark.

Dhar B, Delgado Santander R, Acimovic S Microorganisms. 2024; 12(2).

PMID: 38399780 PMC: 10893025. DOI: 10.3390/microorganisms12020376.

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