The Peculiar Case of the Hyper-thermostable Pyrimidine Nucleoside Phosphorylase from Thermus Thermophilus*

Overview

Journal Chembiochem

Specialty Biochemistry

Date 2020 Dec 1

PMID 33258231

Citations 7

Authors

Felix Kaspar

Peter Neubauer

Anke Kurreck

Affiliations

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Abstract

The poor solubility of many nucleosides and nucleobases in aqueous solution demands harsh reaction conditions (base, heat, cosolvent) in nucleoside phosphorylase-catalyzed processes to facilitate substrate loading beyond the low millimolar range. This, in turn, requires enzymes that can withstand these conditions. Herein, we report that the pyrimidine nucleoside phosphorylase from Thermus thermophilus is active over an exceptionally broad pH (4-10), temperature (up to 100 °C) and cosolvent space (up to 80 % (v/v) nonaqueous medium), and displays tremendous stability under harsh reaction conditions with predicted total turnover numbers of more than 10 for various pyrimidine nucleosides. However, its use as a biocatalyst for preparative applications is critically limited due to its inhibition by nucleobases at low concentrations, which is unprecedented among nonspecific pyrimidine nucleoside phosphorylases.

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