Kallikrein 13 Serves As a Priming Protease During Infection by the Human Coronavirus HKU1

Overview

Journal Sci Signal

Specialties Cell Biology
Physiology
Science

Date 2020 Nov 25

PMID 33234691

Citations 8

Authors

Aleksandra Milewska

Katherine Falkowski

Magdalena Kulczycka

Ewa Bielecka

Antonina Naskalska

Pawel Mak

Adam Lesner

Marek Ochman

Maciej Urlik

Elftherios Diamandis

Ioannis Prassas

Jan Potempa

Tomasz Kantyka

Krzysztof Pyrc

Affiliations

Soon will be listed here.

Abstract

Human coronavirus HKU1 (HCoV-HKU1) is associated with respiratory disease and is prevalent worldwide, but an in vitro model for viral replication is lacking. An interaction between the coronaviral spike (S) protein and its receptor is the primary determinant of tissue and host specificity; however, viral entry is a complex process requiring the concerted action of multiple cellular elements. Here, we found that the protease kallikrein 13 (KLK13) was required for the infection of human respiratory epithelial cells and was sufficient to mediate the entry of HCoV-HKU1 into nonpermissive RD cells. We also demonstrated the cleavage of the HCoV-HKU1 S protein by KLK13 in the S1/S2 region, suggesting that KLK13 is the priming enzyme for this virus. Together, these data suggest that protease distribution and specificity determine the tissue and cell specificity of the virus and may also regulate interspecies transmission.

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