Streamlined Inactivation, Amplification, and Cas13-based Detection of SARS-CoV-2

Overview

Journal Nat Commun

Specialty Biology

Date 2020 Nov 21

PMID 33219225

Citations 187

Authors

Jon Arizti-Sanz

Catherine A Freije

Alexandra C Stanton

Brittany A Petros

Chloe K Boehm

Sameed Siddiqui

Bennett M Shaw

Gordon Adams

Tinna-Solveig F Kosoko-Thoroddsen

Molly E Kemball

Jessica N Uwanibe

Fehintola V Ajogbasile

Philomena E Eromon

Robin Gross

Loni Wronka

Katie Caviness

Lisa E Hensley

Nicholas H Bergman

Bronwyn L MacInnis

Christian T Happi

Jacob E Lemieux

Pardis C Sabeti

Cameron Myhrvold

Affiliations

Soon will be listed here.

Abstract

The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (Streamlined Highlighting of Infections to Navigate Epidemics), a sensitive and specific diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples. We identify the optimal conditions to allow RPA-based amplification and Cas13-based detection to occur in a single step, simplifying assay preparation and reducing run-time. We improve HUDSON to rapidly inactivate viruses in nasopharyngeal swabs and saliva in 10 min. SHINE's results can be visualized with an in-tube fluorescent readout - reducing contamination risk as amplification reaction tubes remain sealed - and interpreted by a companion smartphone application. We validate SHINE on 50 nasopharyngeal patient samples, demonstrating 90% sensitivity and 100% specificity compared to RT-qPCR with a sample-to-answer time of 50 min. SHINE has the potential to be used outside of hospitals and clinical laboratories, greatly enhancing diagnostic capabilities.

Citing Articles

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