The Application of Decellularized Adipose Tissue Promotes Wound Healing

Overview

Journal Tissue Eng Regen Med

Date 2020 Nov 9

PMID 33165769

Citations 8

Authors

Zenan Xia

Xiao Guo

Nanze Yu

Ang Zeng

Loubin Si

Fei Long

Wenchao Zhang

Xiaojun Wang

Lin Zhu

Zhifei Liu

Affiliations

Soon will be listed here.

Abstract

Background: Due to adipose-derived stem cells (ASCs) being easy to obtain, their rapid proliferation rate, and their multidirectional differentiation capabilities, they have been widely used in the field of regenerative medicine. With the progress of decellularized adipose tissue (DAT) and adipose tissue engineering research, the role of DAT in promoting angiogenesis has gradually been emphasized.

Methods: We examined the biological characteristics and biosafety of DAT and evaluated the stem cell maintenance ability and promotion of growth factor secretion through conducting in vitro and in vivo studies.

Results: The tested ASCs showed high rat:es of proliferation and adhered well to DAT. The expression levels of essential genes for cell stem maintenance, including OCT4, SOX2, and Nanog were low at 2-24 h and much higher at 48 and 96 h. The Adipogenic expression level of markers for ASCs proliferation including PPARγ, C/EPBα, and LPL increased from 2 to 96 h. Co-culture of ASCs and DAT increased the secretion of local growth factors, such as VEGF, PDGF-bb, bFGF, HGF, EGF, and FDGF-bb, and secretion gradually increased from 0 to 48 h. A model of full-thickness skin defects on the back of nude mice was established, and the co-culture of ASCs and DAT showed the best in vivo treatment effect.

Conclusion: The application of DAT promotes wound healing, and DAT combined with ASCs may be a promising material in adipose tissue engineering and regenerative medicine.

Citing Articles

Delivery of adipose-derived growth factors from heparinized adipose acellular matrix accelerates wound healing.

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[Research advances of natural biomaterials in promoting wound repair].

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Recent advances in decellularized biomaterials for wound healing.

Xiao H, Chen X, Liu X, Wen G, Yu Y Mater Today Bio. 2023; 19:100589.

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Lee C, Lee Y, Kwon B, Lee S, Ryu Y, Rhie J Tissue Eng Regen Med. 2023; 20(1):59-67.

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