LncRNA UCA1 Affects the Cell Proliferation, Migration, Invasion and Apoptosis of Hepatic Carcinoma Cells by Targeting MicroRNA-193a-3p

Overview

Journal Cancer Manag Res

Publisher Dove Medical Press

Specialty Oncology

Date 2020 Nov 6

PMID 33154669

Citations 2

Authors

Hong-Zhen Wang

Li Liu

Yan Xu

Guang-Ye Zhang

Yan-Yan Wang

Affiliations

Soon will be listed here.

Abstract

Objective/background: Hepatic carcinoma (HCC) is the fourth lethal cancer in the world, but its relationship with lncRNA urothelial cancer-associated 1 (UCA1)/microRNA-193a-3p axis remains unclear, so this study would explore the relationship.

Methods: A real-time polymerase chain reaction (RT-PCR) assay was carried out to quantify lncRNA UCA1 and microRNA-193a-3p in HCC tissues and cells, and relevant overexpression or inhibition vectors were constructed to analyze the influences of lncRNA UCA1 and microRNA-193a-3p on HCC cells. A Transwell assay was used to measure invasion and migration of HCC cells, and a Western blot assay to quantify protein biomarkers of apoptosis, invasion, and migration, a MTT assay to determine cell viability, a flow cytometry to detect cell cycle, and a dual-luciferase reporter gene assay to analyze the correlation between lncRNA UCA1 and microRNA-193a-3p.

Results: LncRNA UCA1 was increased in HCC, while microRNA-193a-3p was decreased. Down-regulated lncRNA UCA1 could up-regulate microRNA-193a-3p, and down-regulated lncRNA UCA1 or up-regulated microRNA-193a-3p would strengthen cell apoptosis and weaken cell migration, invasion, and proliferation. Furthermore, lncRNA UCA1 could negatively regulate microRNA-193a-3p by binding to it.

Conclusion: LncRNA UCA1 promotes malignant hyperproliferation of HCC cells by repressing microRNA-193a-3p.

Citing Articles

A New Understanding of Long Non-Coding RNA in Hepatocellular Carcinoma-From mA Modification to Blood Biomarkers.

Eun J, Cheong J, Jeong J, Kim H Cells. 2023; 12(18).

PMID: 37759495 PMC: 10528438. DOI: 10.3390/cells12182272.

Emerging role of long noncoding RNAs in recurrent hepatocellular carcinoma.

Fang Y, Yang Y, Li N, Zhang X, Huang H World J Clin Cases. 2021; 9(32):9699-9710.

PMID: 34877309 PMC: 8610931. DOI: 10.12998/wjcc.v9.i32.9699.

References

Li Z, Yu D, Li H, Lv Y, Li S . Long non‑coding RNA UCA1 confers tamoxifen resistance in breast cancer endocrinotherapy through regulation of the EZH2/p21 axis and the PI3K/AKT signaling pathway. Int J Oncol. 2019; 54(3):1033-1042. DOI: 10.3892/ijo.2019.4679. View

Petrick J, McGlynn K . The changing epidemiology of primary liver cancer. Curr Epidemiol Rep. 2019; 6(2):104-111. PMC: 6599615. DOI: 10.1007/s40471-019-00188-3. View

Horita K, Kurosaki H, Nakatake M, Kuwano N, Oishi T, Itamochi H . lncRNA UCA1-Mediated Cdc42 Signaling Promotes Oncolytic Vaccinia Virus Cell-to-Cell Spread in Ovarian Cancer. Mol Ther Oncolytics. 2019; 13:35-48. PMC: 6463205. DOI: 10.1016/j.omto.2019.03.003. View

Pang Y, Kartsonaki C, Turnbull I, Guo Y, Chen Y, Clarke R . Adiposity in relation to risks of fatty liver, cirrhosis and liver cancer: a prospective study of 0.5 million Chinese adults. Sci Rep. 2019; 9(1):785. PMC: 6349919. DOI: 10.1038/s41598-018-36460-7. View

Wang F, Ying H, He B, Pan Y, Deng Q, Sun H . Upregulated lncRNA-UCA1 contributes to progression of hepatocellular carcinoma through inhibition of miR-216b and activation of FGFR1/ERK signaling pathway. Oncotarget. 2015; 6(10):7899-917. PMC: 4480724. DOI: 10.18632/oncotarget.3219. View

Wu J, Li W, Ning J, Yu W, Rao T, Cheng F . Long noncoding RNA UCA1 targets miR-582-5p and contributes to the progression and drug resistance of bladder cancer cells through ATG7-mediated autophagy inhibition. Onco Targets Ther. 2019; 12:495-508. PMC: 6331189. DOI: 10.2147/OTT.S183940. View

Salvi A, Conde I, Abeni E, Arici B, Grossi I, Specchia C . Effects of miR-193a and sorafenib on hepatocellular carcinoma cells. Mol Cancer. 2013; 12:162. PMC: 4029516. DOI: 10.1186/1476-4598-12-162. View

Chen D, Lu X, Yang F, Xing N . Circular RNA circHIPK3 promotes cell proliferation and invasion of prostate cancer by sponging miR-193a-3p and regulating MCL1 expression. Cancer Manag Res. 2019; 11:1415-1423. PMC: 6388976. DOI: 10.2147/CMAR.S190669. View

Grewal P, Viswanathen V . Liver cancer and alcohol. Clin Liver Dis. 2012; 16(4):839-50. DOI: 10.1016/j.cld.2012.08.011. View

10.

Xie S, Qin C, Jin L, Wang Q, Shen J, Zhou J . Long noncoding RNA SNHG14 promotes breast cancer cell proliferation and invasion via sponging miR-193a-3p. Eur Rev Med Pharmacol Sci. 2019; 23(6):2461-2468. DOI: 10.26355/eurrev_201903_17393. View

11.

Saitta C, Pollicino T, Raimondo G . Obesity and liver cancer. Ann Hepatol. 2019; 18(6):810-815. DOI: 10.1016/j.aohep.2019.07.004. View

12.

Wang Y, Xie L, Lin J . Gallstones and cholecystectomy in relation to risk of liver cancer. Eur J Cancer Prev. 2018; 28(2):61-67. DOI: 10.1097/CEJ.0000000000000421. View

13.

Tran K, McMenamin U, Coleman H, Cardwell C, Murchie P, Iversen L . Statin use and risk of liver cancer: Evidence from two population-based studies. Int J Cancer. 2019; 146(5):1250-1260. DOI: 10.1002/ijc.32426. View

14.

Yu H, Wang C, Yuan Z, Chen G, Ye T, Yang B . LncRNA NEAT1 promotes the tumorigenesis of colorectal cancer by sponging miR-193a-3p. Cell Prolif. 2018; 52(1):e12526. PMC: 6430453. DOI: 10.1111/cpr.12526. View

15.

Zhu Z, Du S, Yin K, Ai S, Yu M, Liu Y . Knockdown long noncoding RNA nuclear paraspeckle assembly transcript 1 suppresses colorectal cancer through modulating miR-193a-3p/KRAS. Cancer Med. 2018; 8(1):261-275. PMC: 6346262. DOI: 10.1002/cam4.1798. View

16.

Nie W, Ge H, Yang X, Sun X, Huang H, Tao X . LncRNA-UCA1 exerts oncogenic functions in non-small cell lung cancer by targeting miR-193a-3p. Cancer Lett. 2015; 371(1):99-106. DOI: 10.1016/j.canlet.2015.11.024. View

17.

Ma L, Hernandez M, Zhao Y, Mehta M, Tran B, Kelly M . Tumor Cell Biodiversity Drives Microenvironmental Reprogramming in Liver Cancer. Cancer Cell. 2019; 36(4):418-430.e6. PMC: 6801104. DOI: 10.1016/j.ccell.2019.08.007. View

18.

Zhou H, Zhou Y, Feng Z . Long noncoding RNA ZFAS1 promotes hepatocellular carcinoma proliferation by epigenetically repressing miR-193a-3p. Eur Rev Med Pharmacol Sci. 2019; 23(22):9840-9847. DOI: 10.26355/eurrev_201911_19547. View

19.

Ma K, He Y, Zhang H, Fei Q, Niu D, Wang D . DNA methylation-regulated miR-193a-3p dictates resistance of hepatocellular carcinoma to 5-fluorouracil via repression of SRSF2 expression. J Biol Chem. 2011; 287(8):5639-49. PMC: 3285337. DOI: 10.1074/jbc.M111.291229. View

20.

Cui X, Zhao C, Yao X, Qian B, Su C, Ren Y . SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma. RNA Biol. 2018; 15(10):1364-1375. PMC: 6284600. DOI: 10.1080/15476286.2018.1534525. View