Extensive 5'-surveillance Guards Against Non-canonical NAD-caps of Nuclear MRNAs in Yeast

Overview

Journal Nat Commun

Specialty Biology

Date 2020 Nov 3

PMID 33139726

Citations 15

Authors

Yaqing Zhang

David Kuster

Tobias Schmidt

Daniel Kirrmaier

Gabriele Nubel

David Ibberson

Vladimir Benes

Hans Hombauer

Michael Knop

Andres Jaschke

Affiliations

Soon will be listed here.

Abstract

The ubiquitous redox coenzyme nicotinamide adenine dinucleotide (NAD) acts as a non-canonical cap structure on prokaryotic and eukaryotic ribonucleic acids. Here we find that in budding yeast, NAD-RNAs are abundant (>1400 species), short (<170 nt), and mostly correspond to mRNA 5'-ends. The modification percentage of transcripts is low (<5%). NAD incorporation occurs mainly during transcription initiation by RNA polymerase II, which uses distinct promoters with a YAAG core motif for this purpose. Most NAD-RNAs are 3'-truncated. At least three decapping enzymes, Rai1, Dxo1, and Npy1, guard against NAD-RNA at different cellular locations, targeting overlapping transcript populations. NAD-mRNAs are not translatable in vitro. Our work indicates that in budding yeast, most of the NAD incorporation into RNA seems to be disadvantageous to the cell, which has evolved a diverse surveillance machinery to prematurely terminate, decap and reject NAD-RNAs.

Citing Articles

The Mysterious World of Non-Canonical Caps - What We Know and Why We Need New Sequencing Techniques.

Mancini F, Cahova H Chembiochem. 2024; 26(3):e202400604.

PMID: 39248054 PMC: 11823360. DOI: 10.1002/cbic.202400604.

If the 5' cap fits (wear it) - Non-canonical RNA capping.

Potuznik J, Cahova H RNA Biol. 2024; 21(1):1-13.

PMID: 39007883 PMC: 11253889. DOI: 10.1080/15476286.2024.2372138.

Identification and in vitro characterization of UDP-GlcNAc-RNA cap-modifying and decapping enzymes.

Weber F, Motzkus N, Brandl L, Mohler M, Alempijevic A, Jaschke A Nucleic Acids Res. 2024; 52(10):5438-5450.

PMID: 38716860 PMC: 11162767. DOI: 10.1093/nar/gkae353.

Hidden features of NAD-RNA epitranscriptome in life cycle.

Ge S, Wang X, Wang Y, Dong M, Li D, Niu K iScience. 2024; 27(1):108618.

PMID: 38197055 PMC: 10775904. DOI: 10.1016/j.isci.2023.108618.

Identification of NAD-RNA species and ADPR-RNA decapping in Archaea.

Gomes-Filho J, Breuer R, Morales-Filloy H, Pozhydaieva N, Borst A, Paczia N Nat Commun. 2023; 14(1):7597.

PMID: 37989750 PMC: 10663502. DOI: 10.1038/s41467-023-43377-x.

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