"NAD-capQ" Detection and Quantitation of NAD Caps

Overview

Journal RNA

Specialty Molecular Biology

Date 2018 Jul 27

PMID 30045887

Citations 18

Authors

Ewa Grudzien-Nogalska

Jeremy G Bird

Bryce E Nickels

Megerditch Kiledjian

Affiliations

Soon will be listed here.

Abstract

RNA 5' cap structures comprising the metabolic effector nicotinamide adenine dinucleotide (NAD) have been identified in diverse organisms. Here we report a simple, two-step procedure to detect and quantitate NAD-capped RNA, termed "NAD-capQ." By use of NAD-capQ we quantitate NAD-capped RNA levels in , , and human cells, and we measure increases in NAD-capped RNA levels in cells from all three organisms harboring disruptions in their respective "deNADding" enzymes. We further show that NAD-capped RNA levels in human cells respond to changes in cellular NAD concentrations, indicating that NAD capping provides a mechanism for human cells to directly sense and respond to alterations in NAD metabolism. Our findings establish NAD-capQ as a versatile, rapid, and accessible methodology to detect and quantitate 5'-NAD caps on endogenous RNA in any organism.

Citing Articles

Stochastic nature and physiological implications of 5'-NAD RNA cap in bacteria.

Wiedermannova J, Babu R, Yuzenkova Y Nucleic Acids Res. 2024; 52(19):11838-11852.

PMID: 39325642 PMC: 11514452. DOI: 10.1093/nar/gkae813.

The Mysterious World of Non-Canonical Caps - What We Know and Why We Need New Sequencing Techniques.

Mancini F, Cahova H Chembiochem. 2024; 26(3):e202400604.

PMID: 39248054 PMC: 11823360. DOI: 10.1002/cbic.202400604.

Toll/interleukin-1 receptor (TIR) domain-containing proteins have NAD-RNA decapping activity.

Wang X, Yu D, Yu J, Hu H, Hang R, Amador Z Nat Commun. 2024; 15(1):2261.

PMID: 38480720 PMC: 10937652. DOI: 10.1038/s41467-024-46499-y.

Post-transcriptional capping generates coenzyme A-linked RNA.

Sapkota K, Lucas J, Faulkner J, Lichte M, Guo Y, Burke D RNA Biol. 2023; 21(1):1-12.

PMID: 38032240 PMC: 10761072. DOI: 10.1080/15476286.2023.2288740.

Future Perspectives for the Identification and Sequencing of Nicotinamide Adenine Dinucleotide-Capped RNAs.

Mohler M, Jaschke A Acc Chem Res. 2023; 56(21):3000-3009.

PMID: 37852615 PMC: 10634297. DOI: 10.1021/acs.accounts.3c00446.

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