Comparative Lipidomic Analysis of Extracellular Vesicles Derived from APsulloc 331261 Living in Green Tea Leaves Using Liquid Chromatography-Mass Spectrometry

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Nov 3

PMID 33138039

Citations 15

Authors

Hyoseon Kim

Minjung Kim

Kilsun Myoung

Wanil Kim

Jaeyoung Ko

Kwang Pyo Kim

Eun-Gyung Cho

Affiliations

Soon will be listed here.

Abstract

is a popular probiotic species due to its safe and beneficial effects on humans; therefore, novel strains have been isolated and identified from various dietary products. Given that bacteria-derived extracellular vesicles (EVs) have been considered as efficient carriers of bioactive materials and shown to evoke cellular responses effectively, -derived EVs are expected to efficiently elicit health benefits. Herein, we identified APsulloc 331261 living in green tea leaves and isolated EVs from the culture medium. We performed quantitative lipidomic analysis of APsulloc 331261 derived EVs (LEVs) using liquid chromatography-mass spectrometry. In comparison to APsulloc 331261, in LEVs, 67 of 320 identified lipid species were significantly increased and 19 species were decreased. In particular, lysophosphatidylserine(18:4) and phosphatidylcholine(32:2) were critically increased, showing over 21-fold enrichment in LEVs. In addition, there was a notable difference between LEVs and the parent cells in the composition of phospholipids. Our results suggest that the lipidomic profile of bacteria-derived EVs is different from that of the parent cells in phospholipid content and composition. Given that lipids are important components of EVs, quantitative and comparative analyses of EV lipids may improve our understanding of vesicle biogenesis and lipid-mediated intercellular communication within or between living organisms.

Citing Articles

Extracellular vesicles of SLAM216 ameliorate skin symptoms of atopic dermatitis by regulating gut microbiome on serotonin metabolism.

Choi H, Kwak M, Choi Y, Kang A, Mun D, Eor J Gut Microbes. 2025; 17(1):2474256.

PMID: 40028723 PMC: 11881872. DOI: 10.1080/19490976.2025.2474256.

Targeting Inflammation and Skin Aging via the Gut-Skin Axis: The Role of HY7714-Derived Extracellular Vesicles.

Lee H, Lee Y, Hong D, Mo S, Jeon S, Park S Microorganisms. 2025; 12(12.

PMID: 39770669 PMC: 11676968. DOI: 10.3390/microorganisms12122466.

A microbiota-derived metabolite, 3-phenyllactic acid, prolongs healthspan by enhancing mitochondrial function and stress resilience via SKN-1/ATFS-1 in C. elegans.

Kim J, Jo Y, Lim G, Ji Y, Roh J, Kim W Nat Commun. 2024; 15(1):10773.

PMID: 39737960 PMC: 11686233. DOI: 10.1038/s41467-024-55015-1.

Lactic acid bacteria derived extracellular vesicles: emerging bioactive nanoparticles in modulating host health.

Li M, Mao B, Tang X, Zhang Q, Zhao J, Chen W Gut Microbes. 2024; 16(1):2427311.

PMID: 39538968 PMC: 11572086. DOI: 10.1080/19490976.2024.2427311.

Impact of probiotics-derived extracellular vesicles on livestock gut barrier function.

Zhang Y, Song M, Fan J, Guo X, Tao S J Anim Sci Biotechnol. 2024; 15(1):149.

PMID: 39506860 PMC: 11542448. DOI: 10.1186/s40104-024-01102-8.

References

Lee J, Nishiumi S, Yoshida M, Fukusaki E, Bamba T . Simultaneous profiling of polar lipids by supercritical fluid chromatography/tandem mass spectrometry with methylation. J Chromatogr A. 2013; 1279:98-107. DOI: 10.1016/j.chroma.2013.01.020. View

Dang V, Jella K, Ragheb R, Denslow N, Alli A . Lipidomic and proteomic analysis of exosomes from mouse cortical collecting duct cells. FASEB J. 2017; 31(12):5399-5408. PMC: 5690384. DOI: 10.1096/fj.201700417R. View

Dominguez Rubio A, Martinez J, Martinez Casillas D, Coluccio Leskow F, Piuri M, Perez O . BL23 Produces Microvesicles Carrying Proteins That Have Been Associated with Its Probiotic Effect. Front Microbiol. 2017; 8:1783. PMC: 5611436. DOI: 10.3389/fmicb.2017.01783. View

Lee J, Mok H, Lee D, Park S, Ban M, Choi J . UPLC-MS/MS-Based Profiling of Eicosanoids in RAW264.7 Cells Treated with Lipopolysaccharide. Int J Mol Sci. 2016; 17(4):508. PMC: 4848964. DOI: 10.3390/ijms17040508. View

Kaushik J, Kumar A, Duary R, Mohanty A, Grover S, Batish V . Functional and probiotic attributes of an indigenous isolate of Lactobacillus plantarum. PLoS One. 2009; 4(12):e8099. PMC: 2779496. DOI: 10.1371/journal.pone.0008099. View

Nagakubo T, Nomura N, Toyofuku M . Cracking Open Bacterial Membrane Vesicles. Front Microbiol. 2020; 10:3026. PMC: 6988826. DOI: 10.3389/fmicb.2019.03026. View

Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E . Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets. Transfusion. 2014; 55(3):507-21. DOI: 10.1111/trf.12874. View

Benesch M, Ko Y, McMullen T, Brindley D . Autotaxin in the crosshairs: taking aim at cancer and other inflammatory conditions. FEBS Lett. 2014; 588(16):2712-27. DOI: 10.1016/j.febslet.2014.02.009. View

Marco M, Pavan S, Kleerebezem M . Towards understanding molecular modes of probiotic action. Curr Opin Biotechnol. 2006; 17(2):204-10. DOI: 10.1016/j.copbio.2006.02.005. View

10.

Broadhurst D, Goodacre R, Reinke S, Kuligowski J, Wilson I, Lewis M . Guidelines and considerations for the use of system suitability and quality control samples in mass spectrometry assays applied in untargeted clinical metabolomic studies. Metabolomics. 2018; 14(6):72. PMC: 5960010. DOI: 10.1007/s11306-018-1367-3. View

11.

Haraszti R, Didiot M, Sapp E, Leszyk J, Shaffer S, Rockwell H . High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources. J Extracell Vesicles. 2016; 5:32570. PMC: 5116062. DOI: 10.3402/jev.v5.32570. View

12.

Vallejo M, Nakayasu E, Longo L, Ganiko L, Lopes F, Matsuo A . Lipidomic analysis of extracellular vesicles from the pathogenic phase of Paracoccidioides brasiliensis. PLoS One. 2012; 7(6):e39463. PMC: 3382159. DOI: 10.1371/journal.pone.0039463. View

13.

Breil C, Abert Vian M, Zemb T, Kunz W, Chemat F . "Bligh and Dyer" and Folch Methods for Solid-Liquid-Liquid Extraction of Lipids from Microorganisms. Comprehension of Solvatation Mechanisms and towards Substitution with Alternative Solvents. Int J Mol Sci. 2017; 18(4). PMC: 5412294. DOI: 10.3390/ijms18040708. View

14.

Byrd A, Belkaid Y, Segre J . The human skin microbiome. Nat Rev Microbiol. 2018; 16(3):143-155. DOI: 10.1038/nrmicro.2017.157. View

15.

Morita S, Tsuji T, Terada T . Protocols for Enzymatic Fluorometric Assays to Quantify Phospholipid Classes. Int J Mol Sci. 2020; 21(3). PMC: 7037927. DOI: 10.3390/ijms21031032. View

16.

Troost F, van Baarlen P, Lindsey P, Kodde A, de Vos W, Kleerebezem M . Identification of the transcriptional response of human intestinal mucosa to Lactobacillus plantarum WCFS1 in vivo. BMC Genomics. 2008; 9:374. PMC: 2519092. DOI: 10.1186/1471-2164-9-374. View

17.

Yoon J, Lee S, Park Y . Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol. 1998; 48 Pt 1:187-94. DOI: 10.1099/00207713-48-1-187. View

18.

Servin A . Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens. FEMS Microbiol Rev. 2004; 28(4):405-40. DOI: 10.1016/j.femsre.2004.01.003. View

19.

Brinster S, Lamberet G, Staels B, Trieu-Cuot P, Gruss A, Poyart C . Type II fatty acid synthesis is not a suitable antibiotic target for Gram-positive pathogens. Nature. 2009; 458(7234):83-6. DOI: 10.1038/nature07772. View

20.

Cataloluk O, Gogebakan B . Presence of drug resistance in intestinal lactobacilli of dairy and human origin in Turkey. FEMS Microbiol Lett. 2004; 236(1):7-12. DOI: 10.1016/j.femsle.2004.05.010. View