Influence of Beta-lactam Antibiotics and Ciprofloxacin on Cell Envelope of Escherichia Coli

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1987 Jul 1

PMID 3310869

Citations 11

Authors

S Suerbaum

H Leying

H P Kroll

J Gmeiner

W Opferkuch

Affiliations

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Abstract

The effects of subinhibitory concentrations of different beta-lactam antibiotics and one quinolone on the quantitative composition of the outer membrane (OM) of two strains of Escherichia coli, on lipid translocation into the OM, and on the production of capsular K1 polysaccharide were studied. The phospholipid/amino acid ratio was reduced in almost all OM preparations from antibiotic-treated bacteria. In one strain, antibiotic treatment increased the lipopolysaccharide/amino acid ratio. The amount of peptidoglycan fragments bound to the OM was increased by all the antibiotics. In pulse-chase experiments with a radioactive lipid precursor, ciprofloxacin, imipenem, and aztreonam inhibited phospholipid translocation into the OM. Furthermore, imipenem, cephaloridine, and ciprofloxacin induced a pronounced reduction of the production of capsular K1 polysaccharide. Thus, antibiotics seem to induce marked changes of the quantitative composition of the cell envelope of E. coli. Possible connections of these data with findings on the influence of antibiotics on functional parameters of the host-parasite relationship such as OM immunogenicity and serum resistance are discussed.

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References

James R . Identification of an outer membrane protein of Escherichia coli, with a role in the coordination of deoxyribonucleic acid replication and cell elongation. J Bacteriol. 1975; 124(2):918-29. PMC: 235983. DOI: 10.1128/jb.124.2.918-929.1975. View

Braun V, Wolff H . Attachment of lipoprotein to murein (peptidoglycan) of Escherichia coli in the presence and absence of penicillin FL 1060. J Bacteriol. 1975; 123(3):888-97. PMC: 235811. DOI: 10.1128/jb.123.3.888-897.1975. View

Horne D, Hakenbeck R, Tomasz A . Secretion of lipids induced by inhibition of peptidoglycan synthesis in streptococci. J Bacteriol. 1977; 132(2):704-17. PMC: 221914. DOI: 10.1128/jb.132.2.704-717.1977. View

Gmeiner J, Kroll H, MARTIN H . The covalent rigid-layer lipoprotein in cell walls of Proteus mirabilis. Eur J Biochem. 1978; 83(1):227-33. DOI: 10.1111/j.1432-1033.1978.tb12087.x. View

KARKHANIS Y, Zeltner J, Jackson J, Carlo D . A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of Gram-negative bacteria. Anal Biochem. 1978; 85(2):595-601. DOI: 10.1016/0003-2697(78)90260-9. View

Markwell M, Haas S, Bieber L, Tolbert N . A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978; 87(1):206-10. DOI: 10.1016/0003-2697(78)90586-9. View

Bortolussi R, Ferrieri P, Bjorksten B, Quie P . Capsular K1 polysaccharide of Escherichia coli: relationship to virulence in newborn rats and resistance to phagocytosis. Infect Immun. 1979; 25(1):293-8. PMC: 414451. DOI: 10.1128/iai.25.1.293-298.1979. View

Ofek I, Beachey E, Eisenstein B, Alkan M, Sharon N . Suppression of bacterial adherence by subminimal inhibitory concentrations of beta-lactam and aminoglycoside antibiotics. Rev Infect Dis. 1979; 1(5):832-7. DOI: 10.1093/clinids/1.5.832. View

Ruttkowski E, Nixdorff K . Qualitative and quantitative changes in the antibody producing cell response to lipopolysaccharide induced after incorporation of the antigen into bacterial membrane phospholipid vesicles. J Immunol. 1980; 124(6):2548-51. View

10.

Karch H, Nixdorff K . Antibody-producing cell responses to an isolated outer membrane protein and to complexes of this antigen with lipopolysaccharide or with vesicles of phospholipids from Proteus mirabilis. Infect Immun. 1981; 31(3):862-7. PMC: 351398. DOI: 10.1128/iai.31.3.862-867.1981. View

11.

Opal S, Cross A, Gemski P . K antigen and serum sensitivity of rough Escherichia coli. Infect Immun. 1982; 37(3):956-60. PMC: 347631. DOI: 10.1128/iai.37.3.956-960.1982. View

12.

Essig P, MARTIN H, Gmeiner J . Murein and lipopolysaccharide biosynthesis in synchronized cells of Escherichia coli K 12 and the effect of penicillin G, mecillinam and nalidixic acid. Arch Microbiol. 1982; 132(3):245-50. DOI: 10.1007/BF00407959. View

13.

Karch H, Gmeiner J, Nixdorff K . Alteration of the immunoglobulin G subclass responses in mice to lipopolysaccharide: effects of nonbacterial proteins and bacterial membrane phospholipids or outer membrane proteins of Proteus mirabilis. Infect Immun. 1983; 40(1):157-65. PMC: 264830. DOI: 10.1128/iai.40.1.157-165.1983. View

14.

Kroll H, Bhakdi S, Taylor P . Membrane changes induced by exposure of Escherichia coli to human serum. Infect Immun. 1983; 42(3):1055-66. PMC: 264407. DOI: 10.1128/iai.42.3.1055-1066.1983. View

15.

Pluschke G, Mayden J, Achtman M, Levine R . Role of the capsule and the O antigen in resistance of O18:K1 Escherichia coli to complement-mediated killing. Infect Immun. 1983; 42(3):907-13. PMC: 264385. DOI: 10.1128/iai.42.3.907-913.1983. View

16.

Kusecek B, Wloch H, Mercer A, Vaisanen V, Pluschke G, Korhonen T . Lipopolysaccharide, capsule, and fimbriae as virulence factors among O1, O7, O16, O18, or O75 and K1, K5, or K100 Escherichia coli. Infect Immun. 1984; 43(1):368-79. PMC: 263436. DOI: 10.1128/iai.43.1.368-379.1984. View

17.

Bassaris H, Lianou P, Votta E, Papavassiliou J . Effects of subinhibitory concentrations of cefotaxime on adhesion and polymorphonuclear leukocyte function with gram-negative bacteria. J Antimicrob Chemother. 1984; 14 Suppl B:91-6. DOI: 10.1093/jac/14.suppl_b.91. View

18.

Kadurugamuwa J, Anwar H, Brown M, Zak O . Effect of subinhibitory concentrations of cephalosporins on surface properties and siderophore production in iron-depleted Klebsiella pneumoniae. Antimicrob Agents Chemother. 1985; 27(2):220-3. PMC: 176241. DOI: 10.1128/AAC.27.2.220. View

19.

Kadurugamuwa J, Anwar H, Brown M, Zak O . Protein antigens of encapsulated Klebsiella pneumoniae surface exposed after growth in the presence of subinhibitory concentrations of cephalosporins. Antimicrob Agents Chemother. 1985; 28(2):195-9. PMC: 180218. DOI: 10.1128/AAC.28.2.195. View

20.

Dougherty T, Saukkonen J . Membrane permeability changes associated with DNA gyrase inhibitors in Escherichia coli. Antimicrob Agents Chemother. 1985; 28(2):200-6. PMC: 180219. DOI: 10.1128/AAC.28.2.200. View