Further Characterization of Bacillus Subtilis Antibiotic Biosensors and Their Use for Antibacterial Mode-of-action Studies

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2011 Feb 2

PMID 21282422

Citations 3

Authors

Katherine R Mariner

Nicola Ooi

Deborah Roebuck

Alex J ONeill

Ian Chopra

Affiliations

Soon will be listed here.

Abstract

We further examined the usefulness of previously reported Bacillus subtilis biosensors for antibacterial mode-of-action studies. The biosensors could not detect the tRNA synthetase inhibitors mupirocin, indolmycin, and borrelidin, some inhibitors of peptidoglycan synthesis, and most membrane-damaging agents. However, the biosensors confirmed the modes of action of several RNA polymerase inhibitors and DNA intercalators and provided new insights into the possible modes of action of ciprofloxacin, anhydrotetracycline, corralopyronin, 8-hydroxyquinoline, and juglone.

Citing Articles

Chromosome Segregation in Bacillus subtilis Follows an Overall Pattern of Linear Movement and Is Highly Robust against Cell Cycle Perturbations.

El Najjar N, Geisel D, Schmidt F, Dersch S, Mayer B, Hartmann R mSphere. 2020; 5(3).

PMID: 32554717 PMC: 7300352. DOI: 10.1128/mSphere.00255-20.

Genetic validation of aminoacyl-tRNA synthetases as drug targets in Trypanosoma brucei.

Kalidas S, Cestari I, Monnerat S, Li Q, Regmi S, Hasle N Eukaryot Cell. 2014; 13(4):504-16.

PMID: 24562907 PMC: 4000095. DOI: 10.1128/EC.00017-14.

Activity of and development of resistance to corallopyronin A, an inhibitor of RNA polymerase.

Mariner K, McPhillie M, Trowbridge R, Smith C, ONeill A, Fishwick C Antimicrob Agents Chemother. 2011; 55(5):2413-6.

PMID: 21321139 PMC: 3088244. DOI: 10.1128/AAC.01742-10.

References

Suerbaum S, Leying H, Kroll H, Gmeiner J, Opferkuch W . Influence of beta-lactam antibiotics and ciprofloxacin on cell envelope of Escherichia coli. Antimicrob Agents Chemother. 1987; 31(7):1106-10. PMC: 174879. DOI: 10.1128/AAC.31.7.1106. View

Ooi N, Miller K, Hobbs J, Rhys-Williams W, Love W, Chopra I . XF-73, a novel antistaphylococcal membrane-active agent with rapid bactericidal activity. J Antimicrob Chemother. 2009; 64(4):735-40. DOI: 10.1093/jac/dkp299. View

Urban A, Eckermann S, Fast B, Metzger S, Gehling M, Ziegelbauer K . Novel whole-cell antibiotic biosensors for compound discovery. Appl Environ Microbiol. 2007; 73(20):6436-43. PMC: 2075059. DOI: 10.1128/AEM.00586-07. View

Ooi N, Miller K, Randall C, Rhys-Williams W, Love W, Chopra I . XF-70 and XF-73, novel antibacterial agents active against slow-growing and non-dividing cultures of Staphylococcus aureus including biofilms. J Antimicrob Chemother. 2009; 65(1):72-8. DOI: 10.1093/jac/dkp409. View

Boakes S, Appleyard A, Cortes J, Dawson M . Organization of the biosynthetic genes encoding deoxyactagardine B (DAB), a new lantibiotic produced by Actinoplanes liguriae NCIMB41362. J Antibiot (Tokyo). 2010; 63(7):351-8. DOI: 10.1038/ja.2010.48. View

. A guide to sensitivity testing. Report of the Working Party on Antibiotic Sensitivity Testing of the British Society for Antimicrobial Chemotherapy. J Antimicrob Chemother. 1991; 27 Suppl D:1-50. View

Behr W, Honikel K, Hartmann G . Interaction of the RNA polymerase inhibitor chromomycin with DNA. Eur J Biochem. 1969; 9(1):82-92. DOI: 10.1111/j.1432-1033.1969.tb00579.x. View

Fraser R, Creanor J . The mechanism of inhibition of ribonucleic acid synthesis by 8-hydroxyquinoline and the antibiotic lomofungin. Biochem J. 1975; 147(3):401-10. PMC: 1165465. DOI: 10.1042/bj1470401. View

Chao S, Greenleaf A, Price D . Juglone, an inhibitor of the peptidyl-prolyl isomerase Pin1, also directly blocks transcription. Nucleic Acids Res. 2001; 29(3):767-73. PMC: 30403. DOI: 10.1093/nar/29.3.767. View

10.

Wu F, Wu C . Rose Bengal: an inhibitor of ribonucleic acid chain elongation. Biochemistry. 1973; 12(22):4343-8. DOI: 10.1021/bi00746a007. View

11.

Oliva B, ONeill A, Wilson J, OHanlon P, Chopra I . Antimicrobial properties and mode of action of the pyrrothine holomycin. Antimicrob Agents Chemother. 2001; 45(2):532-9. PMC: 90323. DOI: 10.1128/AAC.45.2.532-539.2001. View

12.

Sartiano G, Lynch W, Bullington W . Mechanism of action of the anthracycline anti-tumor antibiotics, doxorubicin, daunomycin and rubidazone: preferential inhibition of DNA polymerase alpha. J Antibiot (Tokyo). 1979; 32(10):1038-45. DOI: 10.7164/antibiotics.32.1038. View

13.

Hurdle J, ONeill A, Chopra I . Prospects for aminoacyl-tRNA synthetase inhibitors as new antimicrobial agents. Antimicrob Agents Chemother. 2005; 49(12):4821-33. PMC: 1315952. DOI: 10.1128/AAC.49.12.4821-4833.2005. View

14.

Oliva B, ONeill A, Miller K, Stubbings W, Chopra I . Anti-staphylococcal activity and mode of action of clofazimine. J Antimicrob Chemother. 2004; 53(3):435-40. DOI: 10.1093/jac/dkh114. View

15.

Higgins D, Chang R, Debabov D, Leung J, Wu T, Krause K . Telavancin, a multifunctional lipoglycopeptide, disrupts both cell wall synthesis and cell membrane integrity in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2005; 49(3):1127-34. PMC: 549257. DOI: 10.1128/AAC.49.3.1127-1134.2005. View

16.

Lindner B, Wiese A, Brandenburg K, Seydel U, Dalhoff A . Lack of interaction of fluoroquinolones with lipopolysaccharides. Antimicrob Agents Chemother. 2002; 46(5):1568-70. PMC: 127130. DOI: 10.1128/AAC.46.5.1568-1570.2002. View

17.

Oliva B, Gordon G, McNicholas P, Ellestad G, Chopra I . Evidence that tetracycline analogs whose primary target is not the bacterial ribosome cause lysis of Escherichia coli. Antimicrob Agents Chemother. 1992; 36(5):913-9. PMC: 188751. DOI: 10.1128/AAC.36.5.913. View

18.

Mukhopadhyay J, Das K, Ismail S, Koppstein D, Jang M, Hudson B . The RNA polymerase "switch region" is a target for inhibitors. Cell. 2008; 135(2):295-307. PMC: 2580802. DOI: 10.1016/j.cell.2008.09.033. View

19.

Hobbs J, Miller K, ONeill A, Chopra I . Consequences of daptomycin-mediated membrane damage in Staphylococcus aureus. J Antimicrob Chemother. 2008; 62(5):1003-8. DOI: 10.1093/jac/dkn321. View

20.

Mariner K, Trowbridge R, Agarwal A, Miller K, ONeill A, Fishwick C . Furanyl-rhodanines are unattractive drug candidates for development as inhibitors of bacterial RNA polymerase. Antimicrob Agents Chemother. 2010; 54(10):4506-9. PMC: 2944609. DOI: 10.1128/AAC.00753-10. View