Random Cloning of Bent DNA Segments from Escherichia Coli Chromosome and Primary Characterization of Their Structures

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1987 Sep 11

PMID 3309887

Citations 30

Authors

T Mizuno

Affiliations

Soon will be listed here.

Abstract

A simple method for the selective detection of DNA segments containing a sequence-directed static bend was developed. Two-dimensional polyacrylamide gel electrophoresis performed at two different temperatures (60 degrees C and 10 degrees C) can effectively separate a bent DNA from a mixture of normal DNA. Using this method, a bank of plasmids carrying bent DNA inserts from the E. coli total chromosome was constructed. The primary characterization of a set of bent DNA segments randomly cloned from E. coli was presented.

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