Three Regions in the DNA of Plasmid PLS1 Show Sequence-directed Static Bending

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1988 Oct 11

PMID 3174448

Citations 8

Authors

J Perez-Martin

G H del Solar

A G de la Campa

M Espinosa

Affiliations

Soon will be listed here.

Abstract

Three regions showing abnormal electrophoretic mobility, which is an indication of the existence of bends in DNA, have been observed in the DNA of plasmid pLS1. These loci have been characterized by assays designed to detect sequence-directed bending in DNA (temperature-dependence migration and two dimensional electrophoresis). The first region (locus B-1) was located within a fragment that contains a proposed inhibitor countertranscribed RNA (RNAII). The second locus (B-2) contains the plasmid plus origin of replication and the third region (locus B-3) was located in the vicinity of a putative antisense RNA (RNAI) of unknown function. The centres of the first two bent DNA regions were located by circular permutation assays at nucleotides 882 (locus B-1) and 634 (locus B-2). The bend centre of locus B-1 was found to be upstream of the promoter for the putative antisense RNAII. The centre of curvature in locus B-2 was located in the vicinity of the putative promoter of the replication proteins RepA and RepB and of a sequence that has three 11-bp direct repeats. The DNA sequence at this region showed the existence of A.T tracts, with an internal repeat of 10 to 11 base pairs, for five helix turns. A complex curvature in the DNA of pLS1 at locus B-2 that may have a regulatory role in plasmid replication is postulated.

Citing Articles

Functional analysis of the stability determinant AlfB of pBET131, a miniplasmid derivative of bacillus subtilis (natto) plasmid pLS32.

Tanaka T J Bacteriol. 2009; 192(5):1221-30.

PMID: 20023009 PMC: 2820838. DOI: 10.1128/JB.01312-09.

Rolling-circle replication of bacterial plasmids.

Khan S Microbiol Mol Biol Rev. 1997; 61(4):442-55.

PMID: 9409148 PMC: 232620. DOI: 10.1128/mmbr.61.4.442-455.1997.

Replication of the promiscuous plasmid pLS1: a region encompassing the minus origin of replication is associated with stable plasmid inheritance.

Del Solar G, Kramer G, Ballester S, Espinosa M Mol Gen Genet. 1993; 241(1-2):97-105.

PMID: 8232217 DOI: 10.1007/BF00280206.

Promoters responsive to DNA bending: a common theme in prokaryotic gene expression.

Perez-Martin J, Rojo F, de Lorenzo V Microbiol Rev. 1994; 58(2):268-90.

PMID: 8078436 PMC: 372964. DOI: 10.1128/mr.58.2.268-290.1994.

Purification and characterization of RepA, a protein involved in the copy number control of plasmid pLS1.

del Solar G, de al Campa A, Perez-Martin J, Choli T, Espinosa M Nucleic Acids Res. 1989; 17(7):2405-20.

PMID: 2497439 PMC: 317632. DOI: 10.1093/nar/17.7.2405.

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