Random Cloning of Bent DNA Segments from Saccharomyces Cerevisiae and Primary Characterization of Their Structures

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1988 Oct 1

PMID 3070349

Citations 1

Authors

T Mizuno

K Itoh

Affiliations

Soon will be listed here.

Abstract

Until recently it was assumed that any short segment of DNA could be approximated as a straight rod. Many instances, however, have been reported in which the helical axis is curved. We have devised a simple method for selective identification of DNA segments containing a sequence-directed bend (curvature), by means of a two-dimensional polyacrylamide gel electrophoresis. In order to gain general insights into the structural features and the functional significance of sequence-directed bends, a bank of plasmids carrying bent DNA inserts from the Saccharomyces cerevisiae total genomic DNA was constructed. Primary characterizations of a set of bent DNA segments randomly cloned from S. cerevisiae are presented. One of the cloned DNA segments appears to be derived from a yeast plasmid, the 2 microns circle DNA.

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Transcription initiation in vivo without classical transactivators: DNA kinks flanking the core promoter of the housekeeping yeast adenylate kinase gene, AKY2, position nucleosomes and constitutively activate transcription.

Angermayr M, Oechsner U, Gregor K, Schroth G, Bandlow W Nucleic Acids Res. 2002; 30(19):4199-207.

PMID: 12364598 PMC: 140550. DOI: 10.1093/nar/gkf551.

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