Detection and Molecular Characterization of and in Free-Ranging Dogs and Ticks from Shahriar County, Tehran Province, Iran

Overview

Journal Iran J Parasitol

Publisher Tehran University of Medical Sciences

Specialty Parasitology

Date 2020 Oct 21

PMID 33082796

Citations 5

Authors

Gholamreza Habibi

Alireza Imani

Asghar Afshari

Soghra Bozorgi

Affiliations

Soon will be listed here.

Abstract

Background: We aimed to detect and characterize vector-borne parasites of and in dog and ticks by PCR assay. Canine babesiosis is a significant tick-borne disease caused by different species. As the infection has not been reported in Shahriar region Tehran, Iran, molecular techniques allowed us to identify tick-borne parasites in asymptomatic dogs.

Methods: The number of 40 dog peripheral blood samples and 27 skin attached ticks were analyzed by molecular PCR assay. The specific primers were used for detecting and .

Results: was detected in 10 dog blood samples (25%). Additionally, infection was identified in 13 dog blood samples (32.5%) and 18 isolated tick DNAs (66.7%). The results of PCR were confirmed by 18S rRNA and Tams1 gene sequence analyzing and have been registered in GenBank under following accession numbers for (MH793502) and (MK105284). The verification of infection in free-ranging dogs and ticks shows dogs might be considered as important natural carriers/reservoirs for in enzootic region for bovine theileriosis. The obtained data may be useful for veterinary practitioners and dog owners to aware of infection in dog and tick to establish the effective preventive measures.

Citing Articles

Canine Babesiosis Caused by Large Species: Global Prevalence and Risk Factors-A Review.

Zygner W, Gojska-Zygner O, Bartosik J, Gorski P, Karabowicz J, Kotomski G Animals (Basel). 2023; 13(16).

PMID: 37627403 PMC: 10451873. DOI: 10.3390/ani13162612.

Genetic diversity of vector-borne pathogens in ixodid ticks infesting dogs from Pakistan with notes on Ehrlichia canis, Rickettsia raoultii and Dirofilaria immitis detection.

Zeb J, Song B, Khan M, Senbill H, Aziz M, Hussain S Parasit Vectors. 2023; 16(1):214.

PMID: 37381006 PMC: 10303367. DOI: 10.1186/s13071-023-05804-2.

Prevalence and Risk Factors Associated with Infection in Two Bovine Portuguese Autochthonous Breeds.

Valente D, Dutra A, Carolino N, Gomes J, Coelho A, Espadinha P Pathogens. 2023; 12(5).

PMID: 37242339 PMC: 10224420. DOI: 10.3390/pathogens12050669.

Canine piroplasmids: Molecular detection and laboratory characterization in dogs from Brasilia, Brazil, with the first molecular evidence of dog exposure to a novel opossum-associated Babesia sp.

de Oliveira C, Yang T, Duarte M, Marr H, McManus C, Andre M Ticks Tick Borne Dis. 2023; 14(4):102181.

PMID: 37084584 PMC: 10698754. DOI: 10.1016/j.ttbdis.2023.102181.

Molecular identification of Babesia canis canis genotype A in a dog from Iran.

Ghasemzade M, Esmaeilnejad B, Asri-Rezaei S, Hadian M Vet Med Sci. 2021; 8(1):21-25.

PMID: 34510799 PMC: 8788953. DOI: 10.1002/vms3.630.

References

Fatemian Z, Salehzadeh A, Sedaghat M, Telmadarraiy Z, Hanafi-Bojd A, Zahirnia A . Hard tick (Acari: Ixodidae) species of livestock and their seasonal activity in Boyer-Ahmad and Dena cities of Kohgiluyeh and Boyer-Ahmad Province, Southwest of Iran. Vet World. 2018; 11(9):1357-1363. PMC: 6200559. DOI: 10.14202/vetworld.2018.1357-1363. View

Solano-Gallego L, Capri A, Pennisi M, Caldin M, Furlanello T, Trotta M . Acute febrile illness is associated with Rickettsia spp infection in dogs. Parasit Vectors. 2015; 8:216. PMC: 4407383. DOI: 10.1186/s13071-015-0824-3. View

Zahler M, Schein E, Rinder H, Gothe R . Characteristic genotypes discriminate between Babesia canis isolates of differing vector specificity and pathogenicity to dogs. Parasitol Res. 1998; 84(7):544-8. DOI: 10.1007/s004360050445. View

Solano-Gallego L, Sainz A, Roura X, Estrada-Pena A, Miro G . A review of canine babesiosis: the European perspective. Parasit Vectors. 2016; 9(1):336. PMC: 4902949. DOI: 10.1186/s13071-016-1596-0. View

Falkeno U, Tasker S, Osterman-Lind E, Tvedten H . Theileria annae in a young Swedish dog. Acta Vet Scand. 2013; 55:50. PMC: 3723473. DOI: 10.1186/1751-0147-55-50. View

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

Irwin P . Canine babesiosis: from molecular taxonomy to control. Parasit Vectors. 2009; 2 Suppl 1:S4. PMC: 2679396. DOI: 10.1186/1756-3305-2-S1-S4. View

Niak A, Anwar M, Khatibi S . Canine babesiosis in Iran. Trop Anim Health Prod. 1973; 5(3):200-1. DOI: 10.1007/BF02251391. View

Fukumoto S, Tamaki Y, Igarashi I, Suzuki H, Xuan X . Immunogenicity and growth inhibitory efficacy of the prime-boost immunization regime with DNA followed by recombinant vaccinia virus carrying the P29 gene of Babesia gibsoni in dogs. Exp Parasitol. 2009; 123(4):296-301. DOI: 10.1016/j.exppara.2009.08.012. View

10.

Inokuma H, Yoshizaki Y, Matsumoto K, Okuda M, Onishi T, Nakagome K . Molecular survey of Babesia infection in dogs in Okinawa, Japan. Vet Parasitol. 2004; 121(3-4):341-6. DOI: 10.1016/j.vetpar.2004.03.012. View

11.

Tavassoli M, Tabatabaei M, Mohammadi M, Esmaeilnejad B, Mohamadpour H . PCR-based Detection of Babesia spp. Infection in Collected Ticks from Cattle in West and North-West of Iran. J Arthropod Borne Dis. 2014; 7(2):132-8. PMC: 3875879. View

12.

Uilenberg G, Franssen F, Perie N, Spanjer A . Three groups of Babesia canis distinguished and a proposal for nomenclature. Vet Q. 1989; 11(1):33-40. DOI: 10.1080/01652176.1989.9694194. View

13.

Schnittger L, Rodriguez A, Florin-Christensen M, Morrison D . Babesia: a world emerging. Infect Genet Evol. 2012; 12(8):1788-809. DOI: 10.1016/j.meegid.2012.07.004. View

14.

Solano-Gallego L, Baneth G . Babesiosis in dogs and cats--expanding parasitological and clinical spectra. Vet Parasitol. 2011; 181(1):48-60. DOI: 10.1016/j.vetpar.2011.04.023. View

15.

Davari B, Alam F, Nasirian H, Nazari M, Abdigoudarzi M, Salehzadeh A . Seasonal distribution and faunistic of ticks in the Alashtar county (Lorestan Province), Iran. Pan Afr Med J. 2017; 27:284. PMC: 5660903. DOI: 10.11604/pamj.2017.27.284.10341. View

16.

Rosa C, Pazzi P, Nagel S, McClure V, Christie J, Troskie M . Theileriosis in six dogs in South Africa and its potential clinical significance. J S Afr Vet Assoc. 2015; 85(1):1114. DOI: 10.4102/jsava.v85i1.1114. View

17.

Criado-Fornelio A, Gonzalez-del-Rio M, Buling-Sarana A, Barba-Carretero J . Molecular characterization of a Babesia gibsoni isolate from a Spanish dog. Vet Parasitol. 2003; 117(1-2):123-9. DOI: 10.1016/j.vetpar.2003.08.006. View

18.

Jongejan F, Uilenberg G . Ticks and control methods. Rev Sci Tech. 1994; 13(4):1201-26. DOI: 10.20506/rst.13.4.818. View

19.

Criado A, Martinez J, Buling A, Barba J, Merino S, Jefferies R . New data on epizootiology and genetics of piroplasms based on sequences of small ribosomal subunit and cytochrome b genes. Vet Parasitol. 2006; 142(3-4):238-47. DOI: 10.1016/j.vetpar.2006.07.004. View

20.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S . MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011; 28(10):2731-9. PMC: 3203626. DOI: 10.1093/molbev/msr121. View