Assessment of the Contribution of Antagonistic Secondary Metabolites to the Antifungal and Biocontrol Activities of NBC275

Overview

Journal Plant Pathol J

Date 2020 Oct 21

PMID 33082733

Citations 8

Authors

Swarnalee Dutta

Sang-Mi Yu

Yong Hoon Lee

Affiliations

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Abstract

An understanding of the contribution of secondary metabolites (SMs) to the antagonistic and biocontrol activities of bacterial biocontrol agents serves to improve biocontrol potential of the strain. In this study, to evaluate the contribution of each SM produced by NBC275 (Pf275) to its antifungal and biocontrol activity, we combined analysis of the genome with our previous study of transposon (Tn) mutants. Thirteen Tn mutants, which belonged to 6 biosynthetic gene clusters (BGCs) of a total 14 BGCs predicted by the antiSMASH tool were identified by the reduction of antifungal activity. The biocontrol performance of Pf275 was significantly dependent on 2,4-diacetylphloroglucinol and pyoverdine. The clusters that encode for arylpolyene and an unidentified small linear lipopeptide influenced antifungal and biocontrol activities. To our knowledge, our study identified the contribution of SMs, such as a small linear lipopeptide and arylpolyene, to biocontrol efficacy for the first time.

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