Posttranscriptional Regulation of 2,4-diacetylphloroglucinol Production by GidA and TrmE in Pseudomonas Fluorescens 2P24

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2014 Apr 22

PMID 24747907

Citations 15

Authors

Wei Zhang

Zhao Zhao

Bo Zhang

Xiao-Gang Wu

Zheng-Guang Ren

Li-Qun Zhang

Affiliations

Soon will be listed here.

Abstract

Pseudomonas fluorescens 2P24 is a soilborne bacterium that synthesizes and excretes multiple antimicrobial metabolites. The polyketide compound 2,4-diacetylphloroglucinol (2,4-DAPG), synthesized by the phlACBD locus, is its major biocontrol determinant. This study investigated two mutants defective in antagonistic activity against Rhizoctonia solani. Deletion of the gidA (PM701) or trmE (PM702) gene from strain 2P24 completely inhibited the production of 2,4-DAPG and its precursors, monoacetylphloroglucinol (MAPG) and phloroglucinol (PG). The transcription of the phlA gene was not affected, but the translation of the phlA and phlD genes was reduced significantly. Two components of the Gac/Rsm pathway, RsmA and RsmE, were found to be regulated by gidA and trmE, whereas the other components, RsmX, RsmY, and RsmZ, were not. The regulation of 2,4-DAPG production by gidA and trmE, however, was independent of the Gac/Rsm pathway. Both the gidA and trmE mutants were unable to produce PG but could convert PG to MAPG and MAPG to 2,4-DAPG. Overexpression of PhlD in the gidA and trmE mutants could restore the production of PG and 2,4-DAPG. Taken together, these findings suggest that GidA and TrmE are positive regulatory elements that influence the biosynthesis of 2,4-DAPG posttranscriptionally.

Citing Articles

A review on mechanisms and prospects of endophytic bacteria in biocontrol of plant pathogenic fungi and their plant growth-promoting activities.

Ali M, Ahmed T, Ibrahim E, Rizwan M, Chong K, Yong J Heliyon. 2024; 10(11):e31573.

PMID: 38841467 PMC: 11152693. DOI: 10.1016/j.heliyon.2024.e31573.

Bioprospecting of multi-stress tolerant Pseudomonas sp. antagonistic to Rhizoctonia solani for enhanced wheat growth promotion.

Kankariya R, Jape P, Patil R, Chaudhari A, Dandi N Int Microbiol. 2024; 28(1):17-35.

PMID: 38581482 DOI: 10.1007/s10123-024-00517-7.

GidA modulates the expression of catalases at the posttranscriptional level and plays a role in virulence.

Srimahaeak T, Thongdee N, Chittrakanwong J, Atichartpongkul S, Jaroensuk J, Phatinuwat K Front Microbiol. 2023; 13:1079710.

PMID: 36726575 PMC: 9884967. DOI: 10.3389/fmicb.2022.1079710.

Identification and Characterization of Bacteria-Derived Antibiotics for the Biological Control of Pea Aphanomyces Root Rot.

Lai X, Niroula D, Burrows M, Wu X, Yan Q Microorganisms. 2022; 10(8).

PMID: 36014014 PMC: 9416638. DOI: 10.3390/microorganisms10081596.

Is Required for Motility, Biofilm Formation, and Stress Response in X2-3.

Zhao D, Wang H, Li Z, Han S, Han C, Liu A Front Microbiol. 2022; 13:840792.

PMID: 35369450 PMC: 8969512. DOI: 10.3389/fmicb.2022.840792.

References

Abbas A, Morrissey J, Marquez P, Sheehan M, Delany I, OGara F . Characterization of interactions between the transcriptional repressor PhlF and its binding site at the phlA promoter in Pseudomonas fluorescens F113. J Bacteriol. 2002; 184(11):3008-16. PMC: 135055. DOI: 10.1128/JB.184.11.3008-3016.2002. View

Reimmann C, Valverde C, Kay E, Haas D . Posttranscriptional repression of GacS/GacA-controlled genes by the RNA-binding protein RsmE acting together with RsmA in the biocontrol strain Pseudomonas fluorescens CHA0. J Bacteriol. 2004; 187(1):276-85. PMC: 538806. DOI: 10.1128/JB.187.1.276-285.2005. View

Duffy B, Defago G . Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains. Appl Environ Microbiol. 1999; 65(6):2429-38. PMC: 91358. DOI: 10.1128/AEM.65.6.2429-2438.1999. View

Bottiglieri M, Keel C . Characterization of PhlG, a hydrolase that specifically degrades the antifungal compound 2,4-diacetylphloroglucinol in the biocontrol agent Pseudomonas fluorescens CHA0. Appl Environ Microbiol. 2006; 72(1):418-27. PMC: 1352262. DOI: 10.1128/AEM.72.1.418-427.2006. View

Meyer S, Scrima A, Versees W, Wittinghofer A . Crystal structures of the conserved tRNA-modifying enzyme GidA: implications for its interaction with MnmE and substrate. J Mol Biol. 2008; 380(3):532-47. DOI: 10.1016/j.jmb.2008.04.072. View

Notz R, Maurhofer M, Schnider-Keel U, Duffy B, Haas D, Defago G . Biotic Factors Affecting Expression of the 2,4-Diacetylphloroglucinol Biosynthesis Gene phlA in Pseudomonas fluorescens Biocontrol Strain CHA0 in the Rhizosphere. Phytopathology. 2008; 91(9):873-81. DOI: 10.1094/PHYTO.2001.91.9.873. View

Bangera M, Thomashow L . Identification and characterization of a gene cluster for synthesis of the polyketide antibiotic 2,4-diacetylphloroglucinol from Pseudomonas fluorescens Q2-87. J Bacteriol. 1999; 181(10):3155-63. PMC: 93771. DOI: 10.1128/JB.181.10.3155-3163.1999. View

Sonnleitner E, Haas D . Small RNAs as regulators of primary and secondary metabolism in Pseudomonas species. Appl Microbiol Biotechnol. 2011; 91(1):63-79. DOI: 10.1007/s00253-011-3332-1. View

Pechy-Tarr M, Bottiglieri M, Mathys S, Lejbolle K, Schnider-Keel U, Maurhofer M . RpoN (sigma54) controls production of antifungal compounds and biocontrol activity in Pseudomonas fluorescens CHA0. Mol Plant Microbe Interact. 2005; 18(3):260-72. DOI: 10.1094/MPMI-18-0260. View

10.

Haas D, Keel C . Regulation of antibiotic production in root-colonizing Peudomonas spp. and relevance for biological control of plant disease. Annu Rev Phytopathol. 2003; 41:117-53. DOI: 10.1146/annurev.phyto.41.052002.095656. View

11.

Phillips D, Fox T, King M, Bhuvaneswari T, Teuber L . Microbial products trigger amino acid exudation from plant roots. Plant Physiol. 2004; 136(1):2887-94. PMC: 523350. DOI: 10.1104/pp.104.044222. View

12.

Couillerot O, Prigent-Combaret C, Caballero-Mellado J, Moenne-Loccoz Y . Pseudomonas fluorescens and closely-related fluorescent pseudomonads as biocontrol agents of soil-borne phytopathogens. Lett Appl Microbiol. 2009; 48(5):505-12. DOI: 10.1111/j.1472-765X.2009.02566.x. View

13.

Kinscherf T, Willis D . Global regulation by gidA in Pseudomonas syringae. J Bacteriol. 2002; 184(8):2281-6. PMC: 134964. DOI: 10.1128/JB.184.8.2281-2286.2002. View

14.

Gong S, Ma Z, Foster J . The Era-like GTPase TrmE conditionally activates gadE and glutamate-dependent acid resistance in Escherichia coli. Mol Microbiol. 2004; 54(4):948-61. DOI: 10.1111/j.1365-2958.2004.04312.x. View

15.

Tian T, Wu X, Duan H, Zhang L . The resistance-nodulation-division efflux pump EmhABC influences the production of 2,4-diacetylphloroglucinol in Pseudomonas fluorescens 2P24. Microbiology (Reading). 2009; 156(Pt 1):39-48. DOI: 10.1099/mic.0.031161-0. View

16.

Ramette A, Moenne-Loccoz Y, Defago G . Polymorphism of the polyketide synthase gene phID in biocontrol fluorescent pseudomonads producing 2,4-diacetylphloroglucinol and comparison of PhID with plant polyketide synthases. Mol Plant Microbe Interact. 2001; 14(5):639-52. DOI: 10.1094/MPMI.2001.14.5.639. View

17.

Cronin D, Moenne-Loccoz Y, Fenton A, Dunne C, Dowling D, OGara F . Role of 2,4-Diacetylphloroglucinol in the Interactions of the Biocontrol Pseudomonad Strain F113 with the Potato Cyst Nematode Globodera rostochiensis. Appl Environ Microbiol. 1997; 63(4):1357-61. PMC: 1389549. DOI: 10.1128/aem.63.4.1357-1361.1997. View

18.

Yang F, Cao Y . Biosynthesis of phloroglucinol compounds in microorganisms--review. Appl Microbiol Biotechnol. 2011; 93(2):487-95. DOI: 10.1007/s00253-011-3712-6. View

19.

Bakker P, Pieterse C, van Loon L . Induced Systemic Resistance by Fluorescent Pseudomonas spp. Phytopathology. 2008; 97(2):239-43. DOI: 10.1094/PHYTO-97-2-0239. View

20.

Haas D, Defago G . Biological control of soil-borne pathogens by fluorescent pseudomonads. Nat Rev Microbiol. 2005; 3(4):307-19. DOI: 10.1038/nrmicro1129. View