Gene Editing in the Ascidian Phallusia Mammillata and Tail Nerve Cord Formation

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2020 Oct 19

PMID 33074543

Citations 1

Authors

Alex McDougall

Celine Hebras

Isa Gomes

Remi Dumollard

Affiliations

Soon will be listed here.

Abstract

Functional approaches for studying embryonic development have greatly advanced thanks to the CRISPR-Cas9 gene editing technique. Previously practiced in just a few organisms, these knockout techniques are now widely applied. Here we describe simple techniques for applying the CRISPR-Cas9 system to study the development of the nerve cord in the ascidian Phallusia mammillata.

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