A Common Aberration with Water-immersion Objective Lenses

Overview

Journal J Microsc

Specialty Radiology

Date 2004 Sep 17

PMID 15369482

Citations 15

Authors

R Arimoto

J M Murray

Affiliations

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Abstract

We describe here an aberration that is frequently encountered with water-immersion but not oil-immersion objectives. The aberration is shown to be induced by tilt of the coverslip out of the plane normal to the optical axis. Model calculations taking into account the path-length distortions introduced by a tilted coverslip satisfactorily reproduce the observed effect on the images of small subresolution fluorescent beads.

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