Butyrate Mediates Anti-inflammatory Effects of in Intestinal Epithelial Cells Through

Overview

Journal Gut Microbes

Specialties Gastroenterology
Microbiology

Date 2020 Oct 15

PMID 33054518

Citations 84

Authors

Marion Lenoir

Rebeca Martin

Edgar Torres-Maravilla

Sead Chadi

Pamela Gonzalez-Davila

Harry Sokol

Philippe Langella

Florian Chain

Luis G Bermudez-Humaran

Affiliations

Soon will be listed here.

Abstract

The commensal bacterium plays a key role in inflammatory bowel disease (IBD) pathogenesis and serves as a general health biomarker in humans. However, the host molecular mechanisms that underlie its anti-inflammatory effects remain unknown. In this study we performed a transcriptomic approach on human intestinal epithelial cells (HT-29) stimulated with TNF-α and exposed to culture supernatant (SN) in order to determine the impact of this commensal bacterium on intestinal epithelial cells. Moreover, modulation of the most upregulated gene after SN contact was validated both and . Our results showed that SN upregulates the expression of , a gene linked to the Wnt/JNK pathway. Interestingly, when we silenced expression, the effect of SN was lost. Butyrate was identified as the effector responsible for modulation. upregulation was also validated in both healthy and inflamed mice treated with either SN or the live bacteria, respectively. Finally, we demonstrated by colon transcriptomics that gut microbiota directly influences expression. This study provides new clues about the host molecular mechanisms involved in the anti-inflammatory effects of the beneficial commensal bacterium .

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