Reference Genes for Real-time PCR Quantification of Messenger RNAs and MicroRNAs in Mouse Model of Obesity

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Jan 28

PMID 24465854

Citations 26

Authors

Petra Matouskova

Hana Bartikova

Iva Bousova

Veronika Hanusova

Barbora Szotakova

Lenka Skalova

Affiliations

Soon will be listed here.

Abstract

Nad(p)h: quinone oxidoreductase, biotransformation enzyme known to be modified by catechins. The effect of selected normalizers for miRNA quantification was tested on two obesity- and diabetes- related miRNAs, miR-221 and miR-29b, respectively. Finally, the combinations of B2M/18S/HPRT1 and miR-16/sno234 were validated as optimal reference genes for mRNA and miRNA quantification in liver and 18S/RPlP0/HPRT1 and sno234/miR-186 in small intestine of MSG mice. These reference genes will be used for mRNA and miRNA normalization in further study of green tea catechins action in obese mice.

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