Dissolved-oxygen Feedback Control Fermentation for Enhancing β-carotene in Engineered Yarrowia Lipolytica

Overview

Journal Sci Rep

Specialty Science

Date 2020 Oct 14

PMID 33051539

Citations 9

Authors

Peng Jun Lv

Shan Qiang

Liang Liu

Ching Yuan Hu

Yong Hong Meng

Affiliations

Soon will be listed here.

Abstract

The DO-stat fed-batch fermentation was carried out to explore the volumetric productivity of β-carotene in engineered Yarrowia lipolytica C11 strain. Using DO-stat fed-batch fermentation, we achieved 94 g/L biomass and 2.01 g/L β-carotene. Both biomass and β-carotene were about 1.28-fold higher than that in fed-batch fermentation. The ATP, NADP/NADPH, and gene expression levels of tHMG, GGS1, carRA, and carB were promoted as compared to that in fed-batch fermentation. As for as the kinetic parameters in DO-stat fed-batch fermentation, μ', Y', and Y' was 0.527, 0.353, and 0.158, respectively. The μ' was elevated 4.66-fold than that in fed-batch fermentation. These data illustrate that more dissolved oxygen increased the biomass. The Y' and Y' were increased 1.15 and 22.57-fold, which suggest that the DO-stat fed-batch fermentation reduced the Crabtree effect and improved the utilization rate of glucose. Therefore, DO-stat fed-batch fermentation is a promising strategy in the industrialized production of β-carotene.

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