Integrated Pathway Engineering and Transcriptome Analysis for Improved Astaxanthin Biosynthesis in

Overview

Journal Synth Syst Biotechnol

Specialty Biotechnology

Date 2022 Sep 12

PMID 36092272

Authors

Dan-Ni Wang

Jie Feng

Chen-Xi Yu

Xin-Kai Zhang

Jun Chen

Liu-Jing Wei

Zhijie Liu

Liming Ouyang

Lixin Zhang

Qiang Hua

Feng Liu

Affiliations

Soon will be listed here.

Abstract

Astaxanthin is a high value carotenoid with a broad range of commercial applications due to its superior antioxidant properties. In this study, β-carotene-producing XK17 constructed in the lab was employed for astaxanthin biosynthesis. The catalytic effects of β-carotene ketolase CrtW and β-carotene hydroxylase CrtZ from various species were investigated. The PspCrtW from sp. and HpCrtZ from were confirmed to be the best combination in converting β-carotene. Several key bottlenecks in biomass and astaxanthin biosynthesis were effectively eliminated by optimizing the expression of the above enzymes and restoring uracil/leucine biosynthesis. In addition, the effects of astaxanthin biosynthesis on cell metabolism were investigated by integrated analysis of pathway modification and transcriptome information. After further optimization, strain DN30 was able to synthesize up to 730.3 mg/L astaxanthin in laboratory 5-L fermenter. This study provides a good metabolic strategy and a sustainable development platform for high-value carotenoid production.

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