LncRNA H19 Inhibits the Progression of Sepsis-Induced Myocardial Injury Via Regulation of the MiR-93-5p/SORBS2 Axis

Overview

Journal Inflammation

Specialties Allergy & Immunology
Pathology

Date 2020 Sep 30

PMID 32996061

Citations 15

Authors

Bin Shan

Jia-Yan Li

Ya-Jiang Liu

Xiao-Bin Tang

Zheng Zhou

Liang-Xian Luo

Affiliations

Soon will be listed here.

Abstract

Sepsis is an infectious disease that seriously endangers human health. It usually leads to myocardial injury which seriously endangers to the health of human beings. H19 has been confirmed to play key roles in various diseases, including sepsis. However, its function in the progression of sepsis-induced myocardial injury remains largely unknown. H9C2 cells were treated with lipopolysaccharide (LPS) to mimic sepsis-induced myocardial injury in vitro. Cell proliferation and apoptosis were detected by MTT assay and flow cytometry, respectively. In addition, gene and protein expression levels in H9C2 cells were measured by quantitative real-time PCR (qRT-PCR) and Western blotting. The levels of inflammatory cytokines in H9C2 cell supernatants were tested by ELISA. JC-1 staining was performed to observe the mitochondrial membrane potential level in H9C2 cells. H19 and SORBS2 were downregulated in H9C2 cells following LPS treatment, while miR-93-5p was upregulated. Moreover, LPS-induced cell growth inhibition and mitochondrial damage were significantly reversed by overexpression of H19. In addition, H19 upregulation notably suppressed LPS-induced inflammatory responses in H9C2 cells. Moreover, H19 sponged miR-93-5p to promote SORBS2 expression. Overall, H19 suppressed sepsis-induced myocardial injury via regulation of the miR-93-5p/SORBS2 axis. H19 attenuated the development of sepsis-induced myocardial injury in vitro via modulation of the miR-93-5p/SORBS2 axis. Thus, H19 could serve as a potential target for the treatment of sepsis-induced myocardial injury.

Citing Articles

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References

Prescott H, Cope T, Gesten F, Ledneva T, Friedrich M, Iwashyna T . Reporting of Sepsis Cases for Performance Measurement Versus for Reimbursement in New York State. Crit Care Med. 2018; 46(5):666-673. PMC: 5899037. DOI: 10.1097/CCM.0000000000003005. View

Faix J . Biomarkers of sepsis. Crit Rev Clin Lab Sci. 2013; 50(1):23-36. PMC: 3613962. DOI: 10.3109/10408363.2013.764490. View

Rello J, Valenzuela-Sanchez F, Ruiz-Rodriguez M, Moyano S . Sepsis: A Review of Advances in Management. Adv Ther. 2017; 34(11):2393-2411. PMC: 5702377. DOI: 10.1007/s12325-017-0622-8. View

Muret K, Klopp C, Wucher V, Esquerre D, Legeai F, Lecerf F . Long noncoding RNA repertoire in chicken liver and adipose tissue. Genet Sel Evol. 2017; 49(1):6. PMC: 5225574. DOI: 10.1186/s12711-016-0275-0. View

Wu Z, Trenner M, Boon R, Spin J, Maegdefessel L . Long noncoding RNAs in key cellular processes involved in aortic aneurysms. Atherosclerosis. 2019; 292:112-118. PMC: 6949864. DOI: 10.1016/j.atherosclerosis.2019.11.013. View

Rao N, Wang X, Xie J, Li J, Zhai Y, Li X . Stem Cells from Human Exfoliated Deciduous Teeth Ameliorate Diabetic Nephropathy In Vivo and In Vitro by Inhibiting Advanced Glycation End Product-Activated Epithelial-Mesenchymal Transition. Stem Cells Int. 2019; 2019:2751475. PMC: 6913167. DOI: 10.1155/2019/2751475. View

Hu H, Wu J, Yu X, Zhou J, Yu H, Ma L . Long non-coding RNA MALAT1 enhances the apoptosis of cardiomyocytes through autophagy inhibition by regulating TSC2-mTOR signaling. Biol Res. 2019; 52(1):58. PMC: 6883637. DOI: 10.1186/s40659-019-0265-0. View

Luo Y, Huang L, Luo W, Ye S, Hu Q . Genomic analysis of lncRNA and mRNA profiles in circulating exosomes of patients with rheumatic heart disease. Biol Open. 2019; 8(12). PMC: 6918777. DOI: 10.1242/bio.045633. View

Xue P, Zhao J, Zheng A, Li L, Chen H, Tu W . Chrysophanol alleviates myocardial injury in diabetic mice by regulating the SIRT1/HMGB1/NF-κB signaling pathway. Exp Ther Med. 2019; 18(6):4406-4412. PMC: 6862128. DOI: 10.3892/etm.2019.8083. View

10.

Xing P, An P, Hu G, Wang D, Zhou M . LncRNA MIAT Promotes Inflammation and Oxidative Stress in Sepsis-Induced Cardiac Injury by Targeting miR-330-5p/TRAF6/NF-κB Axis. Biochem Genet. 2020; 58(5):783-800. DOI: 10.1007/s10528-020-09976-9. View

11.

Zhu Y, Sun A, Meng T, Li H . RETRACTED: Protective role of long noncoding RNA CRNDE in myocardial tissues from injury caused by sepsis through the microRNA-29a/SIRT1 axis. Life Sci. 2020; 255:117849. DOI: 10.1016/j.lfs.2020.117849. View

12.

Hua Q, Chen Y, Liu Y, Li M, Diao Q, Xue H . Circular RNA 0039411 Is Involved in Neodymium Oxide-induced Inflammation and Antiproliferation in a Human Bronchial Epithelial Cell Line via Sponging miR-93-5p. Toxicol Sci. 2019; 170(1):69-81. DOI: 10.1093/toxsci/kfz074. View

13.

Zhang S, He Y, Liu C, Li G, Lu S, Jing Q . miR-93-5p enhances migration and invasion by targeting RGMB in squamous cell carcinoma of the head and neck. J Cancer. 2020; 11(13):3871-3881. PMC: 7171485. DOI: 10.7150/jca.43854. View

14.

Liu J, Jiang M, Deng S, Lu J, Huang H, Zhang Y . miR-93-5p-Containing Exosomes Treatment Attenuates Acute Myocardial Infarction-Induced Myocardial Damage. Mol Ther Nucleic Acids. 2018; 11:103-115. PMC: 5852413. DOI: 10.1016/j.omtn.2018.01.010. View

15.

Yan B, Peng Z, Xing C . SORBS2, mediated by MEF2D, suppresses the metastasis of human hepatocellular carcinoma by inhibitiing the c-Abl-ERK signaling pathway. Am J Cancer Res. 2020; 9(12):2706-2718. PMC: 6943356. View

16.

Zhao L, Wang W, Huang S, Yang Z, Xu L, Yang Q . The RNA binding protein SORBS2 suppresses metastatic colonization of ovarian cancer by stabilizing tumor-suppressive immunomodulatory transcripts. Genome Biol. 2018; 19(1):35. PMC: 5857099. DOI: 10.1186/s13059-018-1412-6. View

17.

Li C, Liu F, Liu S, Pan H, Du H, Huang J . Elevated myocardial SORBS2 and the underlying implications in left ventricular noncompaction cardiomyopathy. EBioMedicine. 2020; 53:102695. PMC: 7058526. DOI: 10.1016/j.ebiom.2020.102695. View

18.

Wang H, Bei Y, Shen S, Huang P, Shi J, Zhang J . miR-21-3p controls sepsis-associated cardiac dysfunction via regulating SORBS2. J Mol Cell Cardiol. 2016; 94:43-53. DOI: 10.1016/j.yjmcc.2016.03.014. View

19.

Guo Y, Ni J, Chen S, Bai M, Lin J, Ding G . MicroRNA-709 Mediates Acute Tubular Injury through Effects on Mitochondrial Function. J Am Soc Nephrol. 2017; 29(2):449-461. PMC: 5791060. DOI: 10.1681/ASN.2017040381. View

20.

Essandoh K, Wang X, Huang W, Deng S, Gardner G, Mu X . Tumor susceptibility gene 101 ameliorates endotoxin-induced cardiac dysfunction by enhancing Parkin-mediated mitophagy. J Biol Chem. 2019; 294(48):18057-18068. PMC: 6885607. DOI: 10.1074/jbc.RA119.008925. View