Species Detection Within the Complex by Novel Probe-Based Real-Time PCRs

Overview

Journal Pathogens

Date 2020 Sep 30

PMID 32993077

Citations 11

Authors

Pavlo Maksimov

Hannes Bergmann

Marion Wassermann

Thomas Romig

Bruno Gottstein

Adriano Casulli

Franz J Conraths

Affiliations

Soon will be listed here.

Abstract

Infections with eggs of () can cause cystic echinococcosis in intermediate host animals and humans. Upon ingestion of viable eggs, oncospheres hatch from the eggs and subsequently develop into fluid-filled larval cysts, most frequently in the liver or the lungs. The slowly growing cysts progressively interfere with organ function. The risk of infection is determined by the host range of the parasite, its pathogenicity and other epidemiologically relevant parameters, which differ significantly among the five species within the complex. It is therefore essential to diagnose the correct species within to help understand specific disease epidemiology and to facilitate effective implementation of control measures. For this purpose, simple, fast and cost-effective typing techniques are needed. We developed quantitative real-time polymerase chain reactions (qPCRs) to target polymorphic regions in the mitochondrial genome of In a single-step typing approach, we distinguished members in four epidemiologically relevant subgroups. These were , , and the cluster. The technique also allowed identification and differentiation of these species from other Echinococcus or Taenia taxa for samples isolated from cysts or faeces.

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