Glutathione-Stabilized Silver Nanoparticles: Antibacterial Activity Against Periodontal Bacteria, and Cytotoxicity and Inflammatory Response in Oral Cells

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2020 Sep 26

PMID 32977686

Citations 12

Authors

Irene Zorraquin-Pena

Carolina Cueva

Dolores Gonzalez de Llano

Begona Bartolome

M Victoria Moreno-Arribas

Affiliations

Soon will be listed here.

Abstract

Silver nanoparticles (AgNPs) have been proposed as new alternatives to limit bacterial dental plaque because of their antimicrobial activity. Novel glutathione-stabilized silver nanoparticles (GSH-AgNPs) have proven powerful antibacterial properties in food manufacturing processes. Therefore, this study aimed to evaluate the potentiality of GSH-AgNPs for the prevention/treatment of oral infectious diseases. First, the antimicrobial activity of GSH-AgNPs against three oral pathogens (, and ) was evaluated. Results demonstrated the efficiency of GSH-AgNPs in inhibiting the growth of all bacteria, especially (IC = 23.64 μg/mL, Ag concentration). Second, GSH-AgNPs were assayed for their cytotoxicity (i.e., cell viability) toward a human gingival fibroblast cell line (HGF-1), as an oral epithelial model. Results indicated no toxic effects of GSH-AgNPs at low concentrations (≤6.16 µg/mL, Ag concentration). Higher concentrations resulted in losing cell viability, which followed the Ag accumulation in cells. Finally, the inflammatory response in the HGF-1 cells after their exposure to GSH-AgNPs was measured as the production of immune markers (interleukins 6 and 8 (IL-6 and IL-8) and tumor necrosis factor-alpha (TNF-α)). GSH-AgNPs activates the inflammatory response in human gingival fibroblasts, increasing the production of cytokines. These findings provide new insights for the use of GSH-AgNPs in dental care and encourage further studies for their application.

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References

Nobbs A, Lamont R, Jenkinson H . Streptococcus adherence and colonization. Microbiol Mol Biol Rev. 2009; 73(3):407-50, Table of Contents. PMC: 2738137. DOI: 10.1128/MMBR.00014-09. View

Esteban-Fernandez A, Ferrer M, Zorraquin-Pena I, Lopez-Lopez A, Moreno-Arribas M, Mira A . In vitro beneficial effects of Streptococcus dentisani as potential oral probiotic for periodontal diseases. J Periodontol. 2019; 90(11):1346-1355. DOI: 10.1002/JPER.18-0751. View

Singh R, Ramarao P . Cellular uptake, intracellular trafficking and cytotoxicity of silver nanoparticles. Toxicol Lett. 2012; 213(2):249-59. DOI: 10.1016/j.toxlet.2012.07.009. View

Rudramurthy G, Swamy M, Sinniah U, Ghasemzadeh A . Nanoparticles: Alternatives Against Drug-Resistant Pathogenic Microbes. Molecules. 2016; 21(7). PMC: 6273897. DOI: 10.3390/molecules21070836. View

Song Y, Guan R, Lyu F, Kang T, Wu Y, Chen X . In vitro cytotoxicity of silver nanoparticles and zinc oxide nanoparticles to human epithelial colorectal adenocarcinoma (Caco-2) cells. Mutat Res. 2015; 769:113-8. DOI: 10.1016/j.mrfmmm.2014.08.001. View

Kampfer A, Urban P, La Spina R, Jimenez I, Kanase N, Stone V . Ongoing inflammation enhances the toxicity of engineered nanomaterials: Application of an in vitro co-culture model of the healthy and inflamed intestine. Toxicol In Vitro. 2019; 63:104738. PMC: 6961208. DOI: 10.1016/j.tiv.2019.104738. View

Marsh P, Head D, Devine D . Ecological approaches to oral biofilms: control without killing. Caries Res. 2015; 49 Suppl 1:46-54. DOI: 10.1159/000377732. View

Correa J, Mori M, Sanches H, da Cruz A, Poiate Jr E, Pola Poiate I . Silver nanoparticles in dental biomaterials. Int J Biomater. 2015; 2015:485275. PMC: 4312639. DOI: 10.1155/2015/485275. View

Mohanta Y, Panda S, Bastia A, Mohanta T . Biosynthesis of Silver Nanoparticles from and Investigation of their Potential Impacts on Food Safety and Control. Front Microbiol. 2017; 8:626. PMC: 5394122. DOI: 10.3389/fmicb.2017.00626. View

10.

Olczak T, Smiga M, Kwiecien A, Bielecki M, Wrobel R, Olczak M . Antimicrobial activity of stable hemiaminals against Porphyromonas gingivalis. Anaerobe. 2017; 44:27-33. DOI: 10.1016/j.anaerobe.2017.01.005. View

11.

Akter M, Sikder M, Rahman M, Ullah A, Hossain K, Banik S . A systematic review on silver nanoparticles-induced cytotoxicity: Physicochemical properties and perspectives. J Adv Res. 2018; 9:1-16. PMC: 6057238. DOI: 10.1016/j.jare.2017.10.008. View

12.

Parnsamut C, Brimson S . Effects of silver nanoparticles and gold nanoparticles on IL-2, IL-6, and TNF-α production via MAPK pathway in leukemic cell lines. Genet Mol Res. 2015; 14(2):3650-68. DOI: 10.4238/2015.April.17.15. View

13.

Inkielewicz-Stepniak I, Santos-Martinez M, Medina C, Radomski M . Pharmacological and toxicological effects of co-exposure of human gingival fibroblasts to silver nanoparticles and sodium fluoride. Int J Nanomedicine. 2014; 9:1677-87. PMC: 3979695. DOI: 10.2147/IJN.S59172. View

14.

Dige I, Nilsson H, Kilian M, Nyvad B . In situ identification of streptococci and other bacteria in initial dental biofilm by confocal laser scanning microscopy and fluorescence in situ hybridization. Eur J Oral Sci. 2007; 115(6):459-67. DOI: 10.1111/j.1600-0722.2007.00494.x. View

15.

Siczek K, Zatorski H, Chmielowiec-Korzeniowska A, Pulit-Prociak J, Smiech M, Kordek R . Synthesis and evaluation of anti-inflammatory properties of silver nanoparticle suspensions in experimental colitis in mice. Chem Biol Drug Des. 2016; 89(4):538-547. DOI: 10.1111/cbdd.12876. View

16.

Oh N, Park J . Endocytosis and exocytosis of nanoparticles in mammalian cells. Int J Nanomedicine. 2014; 9 Suppl 1:51-63. PMC: 4024976. DOI: 10.2147/IJN.S26592. View

17.

Tortella G, Rubilar O, Duran N, Diez M, Martinez M, Parada J . Silver nanoparticles: Toxicity in model organisms as an overview of its hazard for human health and the environment. J Hazard Mater. 2020; 390:121974. DOI: 10.1016/j.jhazmat.2019.121974. View

18.

Kumar P . Oral microbiota and systemic disease. Anaerobe. 2013; 24:90-3. DOI: 10.1016/j.anaerobe.2013.09.010. View

19.

Garcia-Ruiz A, Moreno-Arribas M, Martin-Alvarez P, Bartolome B . Comparative study of the inhibitory effects of wine polyphenols on the growth of enological lactic acid bacteria. Int J Food Microbiol. 2011; 145(2-3):426-31. DOI: 10.1016/j.ijfoodmicro.2011.01.016. View

20.

Kuang X, Chen V, Xu X . Novel Approaches to the Control of Oral Microbial Biofilms. Biomed Res Int. 2019; 2018:6498932. PMC: 6330817. DOI: 10.1155/2018/6498932. View