Ribosome Recycling is Not Critical for Translational Coupling in

Overview

Journal Elife

Specialty Biology

Date 2020 Sep 23

PMID 32965213

Citations 15

Authors

Kazuki Saito

Rachel Green

Allen R Buskirk

Affiliations

Soon will be listed here.

Abstract

We used ribosome profiling to characterize the biological role of ribosome recycling factor (RRF) in . As expected, RRF depletion leads to enrichment of post-termination 70S complexes in 3'-UTRs. We also observe that elongating ribosomes are unable to complete translation because they are blocked by non-recycled ribosomes at stop codons. Previous studies have suggested a role for recycling in translational coupling within operons; if a ribosome remains bound to an mRNA after termination, it may re-initiate downstream. We found, however, that RRF depletion did not significantly affect coupling efficiency in reporter assays or in ribosome density genome-wide. These findings argue that re-initiation is not a major mechanism of translational coupling in . Finally, RRF depletion has dramatic effects on the activity of ribosome rescue factors tmRNA and ArfA. Our results provide a global view of the effects of the loss of ribosome recycling on protein synthesis in

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