Evidence for in Vivo Ribosome Recycling, the Fourth Step in Protein Biosynthesis

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1998 Mar 28

PMID 9463391

Citations 60

Authors

L Janosi

S Mottagui-Tabar

L A ISAKSSON

Y Sekine

E Ohtsubo

S Zhang

S Goon

S NELKEN

M Shuda

A Kaji

Affiliations

Soon will be listed here.

Abstract

Ribosome recycling factor (RRF) catalyzes the fourth step of protein synthesis in vitro: disassembly of the post-termination complex of ribosomes, mRNA and tRNA. We now report the first in vivo evidence of RRF function using 12 temperature-sensitive Escherichia coli mutants which we isolated in this study. At non-permissive temperatures, most of the ribosomes remain on mRNA, scan downstream from the termination codon, and re-initiate translation at various sites in all frames without the presence of an initiation codon. Re-initiation does not occur upstream from the termination codon nor beyond a downstream initiation signal. RRF inactivation was bacteriostatic in the growing phase and bactericidal during the transition between the stationary and growing phase, confirming the essential nature of the fourth step of protein synthesis in vivo.

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