Analysis of TabZIP15 Transcription Factor from Trichoderma Asperellum ACCC30536 and Its Function Under Pathogenic Toxin Stress

Overview

Journal Sci Rep

Specialty Science

Date 2020 Sep 16

PMID 32934312

Authors

Zeyang Yu

Zhiying Wang

Yuzhou Zhang

Yucheng Wang

Zhihua Liu

Affiliations

Soon will be listed here.

Abstract

The TabZIP15 gene encoding a 396 amino acid (aa) polypeptide in the fungus Trichoderma asperellum ACCC30536 was cloned and characterised. The protein includes a basic region motif (NR-x2-QR-x2-R) and has a pillar-like structure. The 25 basic region/leucine zipper transcription factors (TFs) identified in the T. asperellum genome were divided into YAP (14 TFs), ATF2 (5), GCN4 (2), Zip1 (2), BRLZ (1) and u1 (1) subfamilies based on conserved domains. T. asperellum was cultured in minimal media (MM) control, C-Hungry and N-Hungry medium (to simulate nutrient competition and interaction with pathogens, respectively), and differential expression analysis showed that 14 TabZIP genes (including TabZIP15) were significantly altered under both conditions; TabZIP23 responded strongly to N-Hungry media and TabZIP24 responded strongly to C-Hungry media. However, only YAP genes TabZIP15, TabZIP12 and TabZIP2 were significantly upregulated under both conditions, and expression levels of TabZIP15 were highest. T. asperellum was also cultured in the presence of five fungal pathogenic toxins, and RT-qPCR results showed that TabZIP15 was significantly upregulated in four of the five toxin stress conditions (MM + Rhizoctonia solani, MM + Fusarium oxysporum, MM + Alternaria alternata and MM + Cytospora chrysosperma).

References

Shoresh M, Yedidia I, Chet I . Involvement of Jasmonic Acid/Ethylene Signaling Pathway in the Systemic Resistance Induced in Cucumber by Trichoderma asperellum T203. Phytopathology. 2008; 95(1):76-84. DOI: 10.1094/PHYTO-95-0076. View

Mukherjee P, Horwitz B, Herrera-Estrella A, Schmoll M, Kenerley C . Trichoderma research in the genome era. Annu Rev Phytopathol. 2013; 51:105-29. DOI: 10.1146/annurev-phyto-082712-102353. View

Sornaraj P, Luang S, Lopato S, Hrmova M . Basic leucine zipper (bZIP) transcription factors involved in abiotic stresses: A molecular model of a wheat bZIP factor and implications of its structure in function. Biochim Biophys Acta. 2015; 1860(1 Pt A):46-56. DOI: 10.1016/j.bbagen.2015.10.014. View

Thomas D, Jacquemin I . MET4, a leucine zipper protein, and centromere-binding factor 1 are both required for transcriptional activation of sulfur metabolism in Saccharomyces cerevisiae. Mol Cell Biol. 1992; 12(4):1719-27. PMC: 369615. DOI: 10.1128/mcb.12.4.1719-1727.1992. View

Kuras L, Cherest H, Thomas D . A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism. EMBO J. 1996; 15(10):2519-29. PMC: 450184. View

Yin W, Cui P, Wei W, Lin Y, Luo C . Genome-wide identification and analysis of the basic leucine zipper (bZIP) transcription factor gene family in Ustilaginoidea virens. Genome. 2017; 60(12):1051-1059. DOI: 10.1139/gen-2017-0089. View

Yin W, Amaike S, Wohlbach D, Gasch A, Chiang Y, Wang C . An Aspergillus nidulans bZIP response pathway hardwired for defensive secondary metabolism operates through aflR. Mol Microbiol. 2012; 83(5):1024-34. PMC: 3288630. DOI: 10.1111/j.1365-2958.2012.07986.x. View

Hong S, Roze L, Wee J, Linz J . Evidence that a transcription factor regulatory network coordinates oxidative stress response and secondary metabolism in aspergilli. Microbiologyopen. 2013; 2(1):144-60. PMC: 3584220. DOI: 10.1002/mbo3.63. View

Wang X, Wu F, Liu L, Liu X, Che Y, Keller N . The bZIP transcription factor PfZipA regulates secondary metabolism and oxidative stress response in the plant endophytic fungus Pestalotiopsis fici. Fungal Genet Biol. 2015; 81:221-8. DOI: 10.1016/j.fgb.2015.03.010. View

10.

Montibus M, Ducos C, Bonnin-Verdal M, Bormann J, Ponts N, Richard-Forget F . The bZIP transcription factor Fgap1 mediates oxidative stress response and trichothecene biosynthesis but not virulence in Fusarium graminearum. PLoS One. 2013; 8(12):e83377. PMC: 3861502. DOI: 10.1371/journal.pone.0083377. View

11.

Johnson M, Zaretskaya I, Raytselis Y, Merezhuk Y, McGinnis S, Madden T . NCBI BLAST: a better web interface. Nucleic Acids Res. 2008; 36(Web Server issue):W5-9. PMC: 2447716. DOI: 10.1093/nar/gkn201. View

12.

Madeira F, Park Y, Lee J, Buso N, Gur T, Madhusoodanan N . The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic Acids Res. 2019; 47(W1):W636-W641. PMC: 6602479. DOI: 10.1093/nar/gkz268. View

13.

Waterhouse A, Bertoni M, Bienert S, Studer G, Tauriello G, Gumienny R . SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. 2018; 46(W1):W296-W303. PMC: 6030848. DOI: 10.1093/nar/gky427. View

14.

Nordberg H, Cantor M, Dusheyko S, Hua S, Poliakov A, Shabalov I . The genome portal of the Department of Energy Joint Genome Institute: 2014 updates. Nucleic Acids Res. 2013; 42(Database issue):D26-31. PMC: 3965075. DOI: 10.1093/nar/gkt1069. View

15.

Artimo P, Jonnalagedda M, Arnold K, Baratin D, Csardi G, de Castro E . ExPASy: SIB bioinformatics resource portal. Nucleic Acids Res. 2012; 40(Web Server issue):W597-603. PMC: 3394269. DOI: 10.1093/nar/gks400. View

16.

Kumar S, Stecher G, Tamura K . MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016; 33(7):1870-4. PMC: 8210823. DOI: 10.1093/molbev/msw054. View

17.

Bailey T, Boden M, Buske F, Frith M, Grant C, Clementi L . MEME SUITE: tools for motif discovery and searching. Nucleic Acids Res. 2009; 37(Web Server issue):W202-8. PMC: 2703892. DOI: 10.1093/nar/gkp335. View

18.

Ji S, Liu Z, Liu B, Wang Y . Comparative analysis of biocontrol agent Trichoderma asperellum ACCC30536 transcriptome during its interaction with Populus davidiana × P. alba var. pyramidalis. Microbiol Res. 2019; 227:126294. DOI: 10.1016/j.micres.2019.126294. View

19.

Schmittgen T, Livak K . Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008; 3(6):1101-8. DOI: 10.1038/nprot.2008.73. View

20.

Banerjee A, Roychoudhury A . Abscisic-acid-dependent basic leucine zipper (bZIP) transcription factors in plant abiotic stress. Protoplasma. 2015; 254(1):3-16. DOI: 10.1007/s00709-015-0920-4. View